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TitleTarget RNA activates the protease activity of Craspase to confer antiviral defense.
Journal, issue, pagesMol Cell, Vol. 82, Issue 23, Page 4503-44518.e8, Year 2022
Publish dateDec 1, 2022
AuthorsXi Liu / Laixing Zhang / Hao Wang / Yu Xiu / Ling Huang / Zhengyu Gao / Ningning Li / Feixue Li / Weijia Xiong / Teng Gao / Yi Zhang / Maojun Yang / Yue Feng /
PubMed AbstractIn the type III-E CRISPR-Cas system, a Cas effector (gRAMP) is associated with a TPR-CHAT to form Craspase (CRISPR-guided caspase). However, both the structural features of gRAMP and the immunity ...In the type III-E CRISPR-Cas system, a Cas effector (gRAMP) is associated with a TPR-CHAT to form Craspase (CRISPR-guided caspase). However, both the structural features of gRAMP and the immunity mechanism remain unknown for this system. Here, we report structures of gRAMP-crRNA and gRAMP:cRNA:target RNA as well as structures of Craspase and Craspase complexed with cognate target RNA (CTR) or non-cognate target RNA (NTR). Importantly, the 3' anti-tag region of NTR and CTR binds at two distinct channels in Craspase, and CTR with a non-complementary 3' anti-tag induces a marked conformational change of the TPR-CHAT, which allosterically activates its protease activity to cleave an ancillary protein Csx30. This cleavage then triggers an abortive infection as the antiviral strategy of the type III-E system. Together, our study provides crucial insights into both the catalytic mechanism of the gRAMP and the immunity mechanism of the type III-E system.
External linksMol Cell / PubMed:36306795
MethodsEM (single particle)
Resolution2.88 - 3.2 Å
Structure data

33429

7xso

EMDB-33429, PDB-7xso:
Structure of the type III-E CRISPR-Cas effector gRAMP
Method: EM (single particle) / Resolution: 3.01 Å

33430

7xsp

EMDB-33430, PDB-7xsp:
Structure of gRAMP-target RNA
Method: EM (single particle) / Resolution: 2.89 Å

33431

7xsq

EMDB-33431, PDB-7xsq:
Structure of the Craspase
Method: EM (single particle) / Resolution: 2.88 Å

33432

7xsr

EMDB-33432, PDB-7xsr:
Structure of Craspase-target RNA
Method: EM (single particle) / Resolution: 2.97 Å

33433

7xss

EMDB-33433, PDB-7xss:
Structure of Craspase-CTR
Method: EM (single particle) / Resolution: 3.2 Å

33439

7xt4

EMDB-33439, PDB-7xt4:
Structure of Craspase-NTR
Method: EM (single particle) / Resolution: 3.08 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • candidatus scalindua brodae (bacteria)
KeywordsIMMUNE SYSTEM/RNA / IMMUNE SYSTEM-RNA complex

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