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TitleCharacterization of the Escherichia coli pyridoxal 5'-phosphate homeostasis protein (YggS): Role of lysine residues in PLP binding and protein stability.
Journal, issue, pagesProtein Sci., Vol. 31, Page e4471-e4471, Year 2022
Publish dateMar 10, 2022 (structure data deposition date)
AuthorsTramonti, A. / Ghatge, M.S. / Babor, J.T. / Musayev, F.N. / di Salvo, M.L. / Barile, A. / Colotti, G. / Giorgi, A. / Paredes, S.D. / Donkor, A.K. ...Tramonti, A. / Ghatge, M.S. / Babor, J.T. / Musayev, F.N. / di Salvo, M.L. / Barile, A. / Colotti, G. / Giorgi, A. / Paredes, S.D. / Donkor, A.K. / Al Mughram, M.H. / de Crecy-Lagard, V. / Safo, M.K. / Contestabile, R.
External linksProtein Sci. / PubMed:36218140
MethodsX-ray diffraction
Resolution2 - 2.6 Å
Structure data

PDB-7u9c:
Crystal Structure of the wild type Escherichia coli Pyridoxal 5'-phosphate homeostasis protein (YGGS)
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-7u9h:
Crystal Structure of Escherichia coli apo Pyridoxal 5'-phosphate homeostasis protein (YGGS)
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-7uat:
The crystal structure of the K36A mutant of E. coli YGGS in complex with PLP
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-7uau:
The crystal structure of the K137A mutant of E. coli YGGS in complex with PLP
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-7uax:
The crystal structure of the K36A/K38A double mutant of E. coli YGGS in complex with PLP
Method: X-RAY DIFFRACTION / Resolution: 2.07 Å

PDB-7ub4:
The crystal structure of the K36A/K38A/K233A/K234A quadruple mutant of E. coli YGGS in complex with PLP
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-7ub8:
The crystal structure of the K38A/K137A/K233A/K234A quadruple mutant of E. coli YGGS in complex with PLP
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-7ubp:
The crystal structure of the K36A/K137A double mutant of E. coli YGGS in complex with PLP
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-7ubq:
The crystal structure of the wild-type of E. coli YGGS in complex with PNP
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-PLP:
PYRIDOXAL-5'-PHOSPHATE

ChemComp-PO4:
PHOSPHATE ION

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

ChemComp-BU1:
1,4-BUTANEDIOL

ChemComp-PXP:
PYRIDOXINE-5'-PHOSPHATE

Source
  • escherichia coli (E. coli)
KeywordsPROTEIN TRANSPORT / PLP-binding protein / Vitamin B6 / PLP homeostasis

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