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TitleE2/E3-independent ubiquitin-like protein conjugation by Urm1 is directly coupled to cysteine persulfidation.
Journal, issue, pagesEmbo J., Vol. 41, Page e111318-e111318, Year 2022
Publish dateNov 4, 2021 (structure data deposition date)
AuthorsRavichandran, K.E. / Kaduhr, L. / Skupien-Rabian, B. / Shvetsova, E. / Sokolowski, M. / Krutyholowa, R.C. / Kwasna, D. / Brachmann, C. / Lin, S. / Guzman Perez, S. ...Ravichandran, K.E. / Kaduhr, L. / Skupien-Rabian, B. / Shvetsova, E. / Sokolowski, M. / Krutyholowa, R.C. / Kwasna, D. / Brachmann, C. / Lin, S. / Guzman Perez, S. / Wilk, P. / Kosters, M. / Grudnik, P. / Jankowska, U. / Leidel, S.A. / Schaffrath, R. / Glatt, S.
External linksEmbo J. / PubMed:36102610
MethodsX-ray diffraction
Resolution1.1 - 2.5 Å
Structure data

PDB-7q5n:
Crystal structure of Chaetomium thermophilum Ahp1-Urm1 complex
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-7q68:
Crystal structure of Chaetomium thermophilum wild-type Ahp1
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-7q69:
Crystal structure of Chaetomium thermophilum C30S Ahp1 in the pre-reaction state
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-7q6a:
Crystal structure of Chaetomium thermophilum C30S Ahp1 in post-reaction state
Method: X-RAY DIFFRACTION / Resolution: 1.1 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

ChemComp-SO4:
SULFATE ION

Source
  • chaetomium thermophilum (strain dsm 1495 / cbs 144.50 / imi 039719) (fungus)
  • chaetomium thermophilum var. thermophilum dsm 1495 (fungus)
  • chaetomium thermophilum (fungus)
KeywordsLIGASE / urmylation / Urm1 / ubiquitin-like / OXIDOREDUCTASE

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