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Structure paper

TitleStructural insights into the dual activities of the two-barrel RNA polymerase QDE-1.
Journal, issue, pagesNucleic Acids Res., Vol. 50, Page 10169-10186, Year 2022
Publish dateJun 22, 2022 (structure data deposition date)
AuthorsCui, R. / Li, H. / Zhao, J. / Li, X. / Gan, J. / Ma, J.
External linksNucleic Acids Res. / PubMed:36039765
MethodsX-ray diffraction
Resolution2.05 - 2.7 Å
Structure data

PDB-7y7p:
QDE-1 in complex with RNA template, RNA primer and AMPNPP
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-7y7q:
QDE-1 in complex with RNA template, RNA primer and 3'-dGTP
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

PDB-7y7r:
QDE-1 in complex with DNA template, RNA primer and 3'-dGTP
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-7y7s:
QDE-1 in complex with DNA template, RNA primer and AMPNPP
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-7y7t:
QDE-1 in complex with 12nt DNA template, ATP and 3'-dGTP
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-ZAN:
5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]adenosine

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Source
  • neurospora crassa (fungus)
  • synthetic construct (others)
KeywordsRNA BINDING PROTEIN / RNA polymerase / QDE-1 / Complex / RdRP / AMPNPP / 3'-dGTP / DdRP / no primer / ATP

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