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-Structure paper
タイトル | Neuronal RNA granules are ribosome complexes stalled at the pre-translocation state. |
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ジャーナル・号・ページ | J Mol Biol, Vol. 434, Issue 20, Page 167801, Year 2022 |
掲載日 | 2022年10月30日 |
著者 | Kalle Kipper / Abbas Mansour / Arto Pulk / |
PubMed 要旨 | The polarized cell morphology of neurons dictates many neuronal processes, including the axodendridic transport of specific mRNAs and subsequent translation. mRNAs together with ribosomes and RNA- ...The polarized cell morphology of neurons dictates many neuronal processes, including the axodendridic transport of specific mRNAs and subsequent translation. mRNAs together with ribosomes and RNA-binding proteins form RNA granules that are targeted to axodendrites for localized translation in neurons. It has been established that localized protein synthesis in neurons is essential for long-term memory formation, synaptic plasticity, and neurodegeneration. We have used proteomics and electron microscopy to characterize neuronal RNA granules (nRNAg) isolated from rat brain tissues or human neuroblastoma. We show that ribosome-containing RNA granules are morula-like structures when visualized by electron microscopy. Crosslinking-coupled mass-spectrometry identified a potential G3BP2 binding site on the ribosome near the eIF3d-binding site on the 40S ribosomal subunit. We used cryo-EM to resolve the structure of the ribosome-component of nRNAg. The cryo-EM reveals that predominant particles in nRNAg are 80S ribosomes, resembling the pre-translocation state where tRNA's are in the hybrid A/P and P/E site. We also describe a new kind of principal motion of the ribosome, which we call the rocking motion. |
リンク | J Mol Biol / PubMed:36038000 |
手法 | EM (単粒子) |
解像度 | 2.86 - 5.5 Å |
構造データ | EMDB-13954, PDB-7qgg: EMDB-13975: Neuronal RNA granules are ribosome complexes stalled at the pre-translocation state |
化合物 | ChemComp-MG: ChemComp-ZN: ChemComp-HOH: |
由来 |
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キーワード | RIBOSOME / RNA granule / rat |