EMDB-13954: Neuronal RNA granules are ribosome complexes stalled at the pre-t...

Basic information

Entry

Database: EMDB / ID: EMD-13954

• Title: Neuronal RNA granules are ribosome complexes stalled at the pre-translocation state
• Map data: Postprocessed map (Relion) of rat ribosome from neuronal RNA granules.

Sample

• Complex: Ribosome structure in the rat cortex-hippocampus derived neuronal RNA granules.
  • RNA: x 5 types
  • Protein or peptide: x 77 types
• RNA: x 2 types
• Ligand: x 3 types

• Keywords: Ribosome / RNA granule / rat

Function / homology

Function:

regulation of myeloid dendritic cell activation / : / positive regulation of selenocysteine incorporation / Protein hydroxylation / : / mTORC1-mediated signalling / Translesion synthesis by REV1 / Recognition of DNA damage by PCNA-containing replication complex / APC/C:Cdc20 mediated degradation of Cyclin B / APC-Cdc20 mediated degradation of Nek2A / Activated NOTCH1 Transmits Signal to the Nucleus / Downregulation of TGF-beta receptor signaling / Regulation of FZD by ubiquitination / Regulation of TNFR1 signaling / TNFR1-induced NF-kappa-B signaling pathway / Translesion synthesis by POLK / Translesion synthesis by POLI / Termination of translesion DNA synthesis / Gap-filling DNA repair synthesis and ligation in GG-NER / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / Fanconi Anemia Pathway / Regulation of TP53 Degradation / Regulation of TP53 Activity through Methylation / Cyclin D associated events in G1 / Stabilization of p53 / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / ER Quality Control Compartment (ERQC) / Interferon alpha/beta signaling / Endosomal Sorting Complex Required For Transport (ESCRT) / Negative regulators of DDX58/IFIH1 signaling / Activation of IRF3, IRF7 mediated by TBK1, IKKε (IKBKE) / IKK complex recruitment mediated by RIP1 / IRAK2 mediated activation of TAK1 complex / TRAF6-mediated induction of TAK1 complex within TLR4 complex / Alpha-protein kinase 1 signaling pathway / Inactivation of CSF3 (G-CSF) signaling / IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation / Regulation of NF-kappa B signaling / Regulation of TBK1, IKKε (IKBKE)-mediated activation of IRF3, IRF7 / NOD1/2 Signaling Pathway / activated TAK1 mediates p38 MAPK activation / JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 / Formation of Incision Complex in GG-NER / Dual Incision in GG-NER / E3 ubiquitin ligases ubiquitinate target proteins / Translesion Synthesis by POLH / Downregulation of ERBB2:ERBB3 signaling / TCF dependent signaling in response to WNT / Regulation of innate immune responses to cytosolic DNA / HDR through Homologous Recombination (HRR) / Downregulation of ERBB2 signaling / Regulation of signaling by CBL / Ovarian tumor domain proteases / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / DNA Damage Recognition in GG-NER / NOTCH3 Activation and Transmission of Signal to the Nucleus / Interleukin-1 signaling / Downregulation of ERBB4 signaling / Deactivation of the beta-catenin transactivating complex / Negative regulation of MET activity / Translation initiation complex formation / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition / Protein methylation / Regulation of PTEN localization / L13a-mediated translational silencing of Ceruloplasmin expression / SRP-dependent cotranslational protein targeting to membrane / Josephin domain DUBs / Formation of a pool of free 40S subunits / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / 5.8S rRNA binding / RMTs methylate histone arginines / Major pathway of rRNA processing in the nucleolus and cytosol / Pexophagy / cellular response to Thyroid stimulating hormone / Stimuli-sensing channels / N-glycan trimming in the ER and Calnexin/Calreticulin cycle / Metalloprotease DUBs / Regulation of necroptotic cell death / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / Autodegradation of Cdh1 by Cdh1:APC/C / SCF-beta-TrCP mediated degradation of Emi1 / APC/C:Cdc20 mediated degradation of Securin / APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 / Cdc20:Phospho-APC/C mediated degradation of Cyclin A / SCF(Skp2)-mediated degradation of p27/p21 / NRIF signals cell death from the nucleus / NF-kB is activated and signals survival / Autodegradation of the E3 ubiquitin ligase COP1 / Asymmetric localization of PCP proteins / Degradation of DVL / Hedgehog ligand biogenesis / Dectin-1 mediated noncanonical NF-kB signaling / Degradation of GLI1 by the proteasome / Hedgehog 'on' state

Domain/homology:

Ribosomal protein L14, KOW motif / 40S ribosomal protein SA / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ubiquitin-like protein FUBI / Ribosomal protein L30e / Ribosomal protein L2, archaeal-type / Ribosomal L15/L27a, N-terminal / Ribosomal protein L28e / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / metallochaperone-like domain / TRASH domain / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein L1, conserved site / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein L1 signature. / : / Ribosomal protein S12e signature. / Ribosomal protein L1 / Ribosomal protein S12e / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein S2, eukaryotic / S27a-like superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L10e / 40S Ribosomal protein S10 / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / : / Ribosomal protein S7e signature. / Ribosomal protein L44e signature. / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / : / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L19, eukaryotic / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L6e signature. / Ribosomal protein L13e / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein L13e / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S27a / Ribosomal protein S17e, conserved site / : / Ribosomal protein S27a / Ribosomal protein S17e signature. / Ribosomal protein S27a / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3Ae signature. / Ribosomal protein L44e / Ribosomal protein S30 / Ribosomal protein L44 / Ribosomal protein S30 / Ribosomal protein S2, eukaryotic/archaeal / : / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L30e signature 1. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal L40e family / Ribosomal protein S27e signature. / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A

Component:

Large ribosomal subunit protein eL38 / Ribosomal protein L19 / Small ribosomal subunit protein uS14 / 40S ribosomal protein S26 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein RACK1 / Small ribosomal subunit protein uS19 / Ubiquitin-like domain-containing protein / Ribosomal protein L37 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein eS28 / Large ribosomal subunit protein uL14 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein eL43 / 40S ribosomal protein S25 / Ribosomal protein L36a / 40S ribosomal protein S27 / Large ribosomal subunit protein eL39 / 60S ribosomal protein L27 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein eL34 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein eL31 / Small ribosomal subunit protein uS13 / Ribosomal protein L15 / Small ribosomal subunit protein eS17 / Large ribosomal subunit protein eL33 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein eS21 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL18 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein eS19 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein eL29 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein eL13 / Small ribosomal subunit protein eS1 / Large ribosomal subunit protein uL4 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein eS7 / Small ribosomal subunit protein eS8 / Large ribosomal subunit protein eL8 / Small ribosomal subunit protein eS4 / Large ribosomal subunit protein eL20 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein eS24 / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein uL1 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein eS32 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS12 / Small ribosomal subunit protein eS10 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein eL14 / Ubiquitin-ribosomal protein eL40 fusion protein / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein eL22 / 60S ribosomal protein L21 / Large ribosomal subunit protein eL36

• Biological species: Rattus norvegicus (Norway rat)
• Method: single particle reconstruction / cryo EM / Resolution: 2.86 Å
• Authors: Pulk A / Kipper K / Mansour A

Funding support

Estonia, 3 items

Organization	Grant number	Country
European Molecular Biology Organization (EMBO)	IG 3911	Estonia
Estonian Research Council	PSG87	Estonia
Estonian Research Council	MOBTP44	Estonia

• Citation: Journal: J Mol Biol / Year: 2022; Title: Neuronal RNA granules are ribosome complexes stalled at the pre-translocation state.; Authors: Kalle Kipper / Abbas Mansour / Arto Pulk / ; Abstract: The polarized cell morphology of neurons dictates many neuronal processes, including the axodendridic transport of specific mRNAs and subsequent translation. mRNAs together with ribosomes and RNA-binding proteins form RNA granules that are targeted to axodendrites for localized translation in neurons. It has been established that localized protein synthesis in neurons is essential for long-term memory formation, synaptic plasticity, and neurodegeneration. We have used proteomics and electron microscopy to characterize neuronal RNA granules (nRNAg) isolated from rat brain tissues or human neuroblastoma. We show that ribosome-containing RNA granules are morula-like structures when visualized by electron microscopy. Crosslinking-coupled mass-spectrometry identified a potential G3BP2 binding site on the ribosome near the eIF3d-binding site on the 40S ribosomal subunit. We used cryo-EM to resolve the structure of the ribosome-component of nRNAg. The cryo-EM reveals that predominant particles in nRNAg are 80S ribosomes, resembling the pre-translocation state where tRNA's are in the hybrid A/P and P/E site. We also describe a new kind of principal motion of the ribosome, which we call the rocking motion.

History

Deposition	Dec 8, 2021	-
Header (metadata) release	Oct 26, 2022	-
Map release	Oct 26, 2022	-
Update	Oct 23, 2024	-
Current status	Oct 23, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_13954.map.gz / Format: CCP4 / Size: 101 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Postprocessed map (Relion) of rat ribosome from neuronal RNA granules.
• Voxel size: X=Y=Z: 1.41 Å

Density

Contour Level	By AUTHOR: 0.0475
Minimum - Maximum	-0.35740888 - 0.7031244
Average (Standard dev.)	0.0021982058 (±0.023964953)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 298 298 298 Spacing 298 298 298
Cell	A=B=C: 420.18 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_13954_msk_1.map

Additional map: Sharpened map (Phenix) used to RealSpace refine the coordinates.

• File: emd_13954_additional_1.map
• Annotation: Sharpened map (Phenix) used to RealSpace refine the coordinates.

Sample components

Entire : Ribosome structure in the rat cortex-hippocampus derived neuronal...

• Entire: Name: Ribosome structure in the rat cortex-hippocampus derived neuronal RNA granules.

Components

• Complex: Ribosome structure in the rat cortex-hippocampus derived neuronal RNA granules.
  • RNA: RNA (1872-MER)
  • Protein or peptide: 40S ribosomal protein SA
  • Protein or peptide: 40S ribosomal protein S3a
  • Protein or peptide: 40S ribosomal protein S3
  • Protein or peptide: 40S ribosomal protein S4, X isoform
  • Protein or peptide: 40S ribosomal protein S5
  • Protein or peptide: 40S ribosomal protein S7
  • Protein or peptide: 40S ribosomal protein S8
  • Protein or peptide: 40S ribosomal protein S10
  • Protein or peptide: 40S ribosomal protein S11
  • Protein or peptide: 40S ribosomal protein S15
  • Protein or peptide: Rps16 protein
  • Protein or peptide: 40S ribosomal protein S17
  • Protein or peptide: 40S ribosomal protein S18
  • Protein or peptide: 40S ribosomal protein S19
  • Protein or peptide: 40S ribosomal protein S20
  • Protein or peptide: 40S ribosomal protein S21
  • Protein or peptide: 40S ribosomal protein S23
  • Protein or peptide: 40S ribosomal protein S26
  • Protein or peptide: 40S ribosomal protein S28
  • Protein or peptide: 40S ribosomal protein S29
  • Protein or peptide: Receptor of activated protein C kinase 1
  • Protein or peptide: 40S ribosomal protein S2
  • Protein or peptide: 40S ribosomal protein S6
  • Protein or peptide: 40S ribosomal protein S9
  • Protein or peptide: 40S ribosomal protein S12
  • Protein or peptide: 40S ribosomal protein S13
  • Protein or peptide: 40S ribosomal protein S14
  • Protein or peptide: 40S ribosomal protein S15a
  • Protein or peptide: 40S ribosomal protein S24
  • Protein or peptide: 40S ribosomal protein S25
  • Protein or peptide: 40S ribosomal protein S27
  • Protein or peptide: Ubiquitin-like domain-containing protein
  • Protein or peptide: Ubiquitin-40S ribosomal protein S27a
  • Protein or peptide: 60S ribosomal protein L8
  • Protein or peptide: 60S ribosomal protein L3
  • Protein or peptide: 60S ribosomal protein L4
  • RNA: RNA (157-MER)
  • RNA: RNA (121-MER)
  • Protein or peptide: 60S ribosomal protein L5
  • Protein or peptide: 60S ribosomal protein L6
  • Protein or peptide: 60S ribosomal protein L7
  • Protein or peptide: 60S ribosomal protein L7a
  • Protein or peptide: 60S ribosomal protein L9
  • Protein or peptide: 60S ribosomal protein L10
  • Protein or peptide: 60S ribosomal protein L11
  • Protein or peptide: 60S ribosomal protein L13
  • Protein or peptide: 60S ribosomal protein L14
  • Protein or peptide: Ribosomal protein L15
  • Protein or peptide: 60S ribosomal protein L13a
  • Protein or peptide: 60S ribosomal protein L17
  • Protein or peptide: 60S ribosomal protein L18
  • Protein or peptide: Ribosomal protein L19
  • Protein or peptide: 60S ribosomal protein L18a
  • Protein or peptide: 60S ribosomal protein L21
  • Protein or peptide: 60S ribosomal protein L22
  • Protein or peptide: 60S ribosomal protein L23
  • Protein or peptide: 60S ribosomal protein L24
  • Protein or peptide: 60S ribosomal protein L23a
  • Protein or peptide: 60S ribosomal protein L26
  • Protein or peptide: 60S ribosomal protein L27
  • Protein or peptide: 60S ribosomal protein L27a
  • Protein or peptide: 60S ribosomal protein L29
  • Protein or peptide: 60S ribosomal protein L30
  • Protein or peptide: 60S ribosomal protein L31
  • Protein or peptide: 60S ribosomal protein L32
  • Protein or peptide: 60S ribosomal protein L35a
  • Protein or peptide: 60S ribosomal protein L34
  • Protein or peptide: 60S ribosomal protein L35
  • Protein or peptide: 60S ribosomal protein L36
  • Protein or peptide: Ribosomal protein L37
  • Protein or peptide: 60S ribosomal protein L38
  • Protein or peptide: 60S ribosomal protein L39
  • Protein or peptide: Ubiquitin-60S ribosomal protein L40
  • Protein or peptide: 60S ribosomal protein L41
  • Protein or peptide: Ribosomal protein L36a
  • Protein or peptide: 60S ribosomal protein L37a
  • Protein or peptide: 60S ribosomal protein L28
  • RNA: RNA (4803-MER)
  • RNA: RNA (5'-D(P*()P*()P*()P*())-R(P*UP*UP*AP*CP*GP*GP*CP*GP*GP*UP*()P*()P*()P*()P*()P*())-3')
  • Protein or peptide: 60S ribosomal protein L10a
  • Protein or peptide: ALA-ALA-LYS-ALA
• RNA: RNA (76-MER)
• RNA: RNA (76-MER)
• Ligand: MAGNESIUM ION
• Ligand: ZINC ION
• Ligand: water

Supramolecule #1: Ribosome structure in the rat cortex-hippocampus derived neuronal...

• Supramolecule: Name: Ribosome structure in the rat cortex-hippocampus derived neuronal RNA granules.; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#79, #82-#84
• Source (natural): Organism: Rattus norvegicus (Norway rat) / Organ: Brain / Tissue: Cortex and hippocampus / Organelle: RNA granule

Macromolecule #1: RNA (1872-MER)

• Macromolecule: Name: RNA (1872-MER) / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 604.241188 KDa

Sequence

String:

UACCUGGUUG AUCCUGCCAG UAGCAU(A2M)UGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGU ACA GUGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGU(OMU)CC(PSU) U(OMU)GGUCGCUC GCUCCUCUCC UACUU GGAU AACUGUGGUA (A2M)UUCUAG(A2M)GC UAAUA(OMC)AUGC CGACGGGCGC UGACCCCCCU UCCCGCGGGG GGGA UGCGU GCAUUUAUCA GAUCAAAACC AACCCGGUCA GCCCCCUCCC GGCUCCGGCC GGGGGUCGGG CGCCGGCGGC UUUGG UGAC UCUAGAUAAC CUCGGGCCGA UCGCACGCCC UCCGUGGCGG CGACGACCCA UUCGAACGUC UGCCCUAUCA ACUUUC GAU GGUAGUCGCC GUGCCUACCA UGGUGACCAC GGGUGACGGG GAAUCAGGGU UCGAUUCCGG AGAGGGAGCC UGAGAAA CG GCUACCACAU CCAAGGAAGG CAGCAGGCGC GC(A2M)AAUUACC CACUCCCGAC CCGGGGA(OMG)GU AGUGA(OMC) GAA AAAUAACAAU ACAGGACUCU UUCGAGGCCC UGUAAUUGGA AUGAGUC(MMX)AC UUUAAAUCCU UUAACGAGGA UCC AUUGGA GGGCAAGUCU GG(PSU)GCCAGCA GCCGCGGUAA UUCCAGCUCC AAUA(OMG)CGUAU AUUAAAGUUG CUGCAG UU(A2M) AAAAGCUCGU AGUU(OMG)GAUCU UGGGAGCGGG CGGGCGGUCC GCCGCGAGGC GAGUCACCGC CCGUCCCCG CCCCUUGCCU CUCGGCGCCC CCUCGAUGCU CUUAGCUGAG UGUCCCGCGG GGCCCGAAGC GUUUACUUUG AAAAAA (5MU)UA GAGUG(PSU)(PSU)CAA AGCAGGCCCG AGCCGCCUGG AUACCGCAGC UAGGAAUAAU GGAAUAGGAC CGCGG UUCU AUUUUGUUGG UUUUCGGAAC UGAGGCCAUG AUUAAGAGGG ACGGCCGGGG GCAUUCGUAU UGCGCCGCUA GAGGUG AAA UUCUUGGACC GGCGCAAGAC GGACCAGAGC GAAAGCAUUU GCCAAGAAUG UUUUCAUUAA UCAAGA(A2M)CGA AAG UCGGAG GUUCGAAGAC GAUCAGAUAC CGUCGUAGUU CCGACCA(PSU)AA ACGAUGCCGA CUGGCGAUGC GGCGGCGUUA UUCCCAUGA CCCGCCGGGC AGCUUCCGGG AAACCAAAGU CUUUGGGUUC CGGGGGGAGU AUGGUUGCAA AGCUGAAACU U AAAGGAAU UGACGGAAGG GCACCACCAG GAGUGGAGCC UGCGGCUUAA U(PSU)UGAC(B8N)CAA CACGGGAAAC CUCA CCCGG CCCGGACACG GACAGGAUUG ACAGAUUGAU AGCUCUUUCU CGAUUCCGUG GGUGGUGGUG CAUGGC(4AC)GUU C UUAGUUGG UGGAGCGAUU UGUCUGGUUA AUUC(5MC)GAUAA CGAACGAGAC UCUGGCAUGC UAACUAGUUA CGCGACCC C CGAGCGGUCG GCGUCCCCCA ACUUCUUAGA GGGACAAGUG GCGUUCAGCC ACCCGAGAUU GAGCAAUAAC AGGUCUGUG AUGCCCUUAG AUGUCCGGGG CUGCACGCGC GCUACACUGA CUGGCUCAGC GUGUGCCUAC CCUACGCCGG CAGGCGCGGG UAACCCGUU GAACCCCAUU CGUGAUGGGG AUCGGGGAUU GCAAUUAUUC CCCAUGAACG AGGAAUUCCC AGUAAGUGCG G GUCAUAAG CUUGCGUUGA UU(A2M)AGUCCCU GCCCUUUGUA CACACCG(OMC)CC GUCG(OMC)UACUA CCGAUUGGAU GGUUUAGUG AGGCCCUCGG AUCGGCCCCG CCGGGGUCGG CCCACGGCCC UGGCGGAGCG CUGAGAAGAC GGUCGAACUU G (M7A)CUAUCUA GAGGAAGUAA AAGUCG(UR3)A(6MZ)C AAGGUUUC(4AC)G UAGGUG(MA6)(MA6)CC UGCGGAAG G AUCAUUA

Macromolecule #38: RNA (157-MER)

• Macromolecule: Name: RNA (157-MER) / type: rna / ID: 38 / Number of copies: 1
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 50.449812 KDa

Sequence

String:

CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGA CACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGAG CGUCGCUU

GENBANK: GENBANK: X00722.1

Macromolecule #39: RNA (121-MER)

• Macromolecule: Name: RNA (121-MER) / type: rna / ID: 39 / Number of copies: 1
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 38.998078 KDa

Sequence

String:

GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU UU

GENBANK: GENBANK: X83746.1

Macromolecule #79: RNA (4803-MER)

• Macromolecule: Name: RNA (4803-MER) / type: rna / ID: 79 / Number of copies: 1
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 1.5539542499999999 MDa

Sequence

String:

CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC GCGCGCCGCG GCGCGGGAAA UGUGGCGUAC G GAAGACCC ACUCCCCGGC GCCGCUCGUG GGGGGCCCAA GUCCUUCUGA UCGAGGCCCA GCCCGUGGAC GGUGUGAGGC CG GUAGCGG CCCCCGGCGC GCCGGGCCCG GGUCUUCCCG GAGUCGGGUU GCUUGGGAAU GCAGCCCAAA GCGGGUGGUA AAC UCCAUC UAAGGCUAAA UACCGGCACG AGACCGAUAG CCAACAAGUA CCGUAAGGGA AAGUUGAAAA GAACUUUGAA GAGA GAGUU CAAGAGGGCG UGAAACCGUU AAGAGGUAAA CGGGUGGGGU CCGCGCAGUC CGCCCGGAGG AUUCAACCCG GCGGC GCGC GUCCGGCCGU GCCGGUGGUC CCGGCGGAUC UUUCCCGCUC CCCGUUCCUC CCGACCCCUC CACCCGCGCG UCUCUC UCC CCCCUCCCCG CGUCCCGCCG UCGCCGUCCC CGCUCCUCCC UCCGGGGGGG UGUCGGCGGG CGCUCCGGCG GCGGACG CG GGGUGUGGUG GGGGCGCGCG GGCGGGGCCG GGGGUGGUGU CGGCGGGGGA CCGCCCCCCG GAUCGGCGAC CGGCCGCC G CCGGGCGCAC UUCCACCGUG GCGGUGCGCC GCGACCGGCU CCGGGACGGC UGGGAAGGCC CGGCGGGGAA GGUGGCUCG GGGGGGGCGG CGUCACCCGU GGGCGCCGGA CCACCCCGCC CCGAGUGUUA CAGCCCCCCG GCAGCAGCGC UCGCCGAAUC CCGGGGCCG AGGGAGCCGG AUACCCGUCG CCGCGCUCUC CCCCCGGCCU CUCCCCUCCC GCCCCUCCCC GUGGGGUGGC G GAAAGGGG GGCGGUCGCG GGGGCCGGGC CGCCCCUCCC ACGGCGCGAC CGCUCUCCCA CCCCCCGUCG CCUCCGUCGU CC CUCUCGG GGGUCCGGGG GCCCGGGGGG CGGGGCGGAC UGUCCCCAGU GCGCCCCGGG CGUCGUCGCG CCGUCGGGCC CGG GGGGGC CGUCGUCACG CGCUCUCCCU CCCCUUCUCG GGGUGGGGGG GAGCGAAGCC GAGCGCACGG GGUCGGCGGC GAUG UCGGC UACCCACCCG ACCCGUCUUG AAACACGGAC CAAGGAGUCU AACGCGUGCG CGAGUCAGGG GCUCGUCCGA AAGCC GCCG UGGCGCAAUG AAGGUGAAGG GCCCCGUUCC CGGGGGCCCC GAGGUGGGAU CCCGAGGCCU CUCCAGUCCG CCGAGG GCG CACCACCGGC CCGUCUCGCC CGCCGCGCCG GGGAGGUGGA GCACGAGCGU ACGCGUUAGG ACCCGAAAGA UGGUGAA CU AUGCCUGGGC AGGGCGAAGC CAGAGGAAAC UCUGGUGGAG GUCCGUAGCG GUCCUGACGU GCAAAUCGGU CGUCCGAC C UGGGUAUAGG GGCGAAAGAC UAAUCGAACC AUCUAGUAGC UGGUUCCCUC CGAAGUUUCC CUCAGGAUAG CUGGCGCUC UCGCAACGCG GUCGCUCGAC AACCCGCAGU UUUAUCCGGU AAAGCGAAUG AUUAGAGGUC UUGGGGCCGA AACGAUCUCA ACCUAUUCU CAAACUUUAA AUGGGUAAGA AGCCCGGCUC GCUGGCGUGG AGCCGGGCGU GGAAUGCGAG UGCCUAGUGG G CCACUUUU GGUAAGCAGA ACUGGCGCUG CGGGAUGAAC CGAACGCCGG GUUAAGGCGC CCGAUGCCGA CGCUCAUCAG AC CCCAGAA AAGGUGUUGG UUGAUAUAGA CAGCAGGACG GUGGCCAUGG AAGUCGGAAU CCGCUAGGAG UGUGUAACAA CUC ACCUGC CGAAUCAACU AGCCCUGAAA AUGGAUGGCG CUGGAGCGUC GGGCCCAUAC CCGGCCGUCG CCGGCAGUCG GAAC GGGAC GGGAGCGGCC GCGGGCGCGC GACCCCGGGG GCCGGGCGGC GUCGGCUUCG GCCGGCCGCC GCCCGUCCAC CCCCG GGGC UCCCCCCGCG GCGUCGGGCC CCGCGGACGC UACGCCGCGA CGAGUAGGAG GGCCGCUGCG GUGAGCCUUG AAGCCU AGG GCGCGGGCCC GGGUGGAGCC GCCGCAGGUG CAGAUCUUGG UGGUAGUAGC AAAUAUUCAA ACGAGAACUU UGAAGGC CG AAGUGGAGAA GGGUUCCAUG UGAACAGCAG UUGAACAUGG GUCAGUCGGU CCUGAGAGAU GGGCGAGUGC CGUUCCGA A GGGACGGGCG AUGGCCUCCG UUGCCCUCAG CCGAUCGAAA GGGAGUCGGG UUCAGAUCCC CGAAUCCGGA GUGGCGGAG AUGGGCGCCG CGAGGCGUCC AGUGCGGUAA CGCGACCGAU CCCGGAGAAG CCGGCGGGAG CCCCGGGGAG AGUUCUCUUU UCUUUGUGA AGGGCAGGGC GCCCUGGAAU GGGUUCGCCC CGAGAGAGGG GCCCGUGCCU UGGAAAGCGU CGCAGUUCCG G CGGCGUCC GGUGAGCUCU CGCUGGCCCU UGAAAAUCCG GGGGAGAGGG UGUAAAUCUC GCGCCGGGCC GUACCCAUAU CC GCAGCAG GUCUCCAAGG UGAACAGCCU CUGGCAUGUU GGAACAAUGU AGGUAAGGGA AGUCGGCAAG CCGGAUCCGU AAC UUCGGG AUAAGGAUUG GCUCUAAGGG CUGGGUCGGU CGGGCUGGGG CGCGAAGGCG GGGCUGGGCG CGCGCCGCGG CUGG ACGAG GCGCCGCCGC CCCCCUCCCA CGUCCGGGGA GACCCCCUCC UUUCCGCCCG GGCCCGCCCU CCCCUCUCCC CGCGG GGCC CCGCCGUCCC CCGCGUCGUC GCCGUGGUCC CCUCCUCCCU CCCUUCUUCC CCGUCCGCGG GGGGGACGGG GCGGGU GCG GGGGGGCGCG CGCGCGCGCG GCCCAGGGGC GGCGGGUCCA ACCCCGCGCG GGCCGGAGCG GGGGGAACCC GCGGGCC CC CGGUGGGGGG GGGCCCGGAC ACCCGGGGGG GACCGGCGGC GGCGGCGACU CUGGACGCGA GCCGGGCCCU UCCCGUGG A UCGCCCCAGC UGCGGCGGGC GUCGCGGCCG CUCCCGGGGA GCCCGGCGGG UCGCCGGCGC GGGGUUUUCC UCCGGCCUC GUCCUCCCCC UUCCCCCUCC GCGGGGUCGG GGGUUCCCGG GGUUCGGGGU UCUCCUCCGC GUCGGCGGUU CCCCCGCCGG GUGCGCCCC CCGGGCCGCG GUUUCCCGCG CGGCGCCUCG CCUCGGCCGG CGCCUAGCAG CCGACUUAGA ACUGGUGCGG A CCAGGGGA AUCCGACUGU UUAAUUAAAA CAAAGCAUCG CGAAGGCCCG CGGCGGGUGU UGACGCGAUG UGAUUUCUGC CC AGUGCUC UGAAUGUCAA AGUGAAGAAA UUCAAUGAAG CGCGGGUAAA CGGCGGGAGU AACUAUGACU CUCUUAAGGU AGC CAAAUG CCUCGUCAUC UAAUUAGUGA CGCGCAUGAA UGGAUGAACG AGAUUCCCAC UGUCCCUACC UACUAUCCAG CGAA ACCAC AGCCAAGGGA ACGGGCUUGG CGGAAUCAGC GGGGAAAGAA GACCCUGUUG AGCUUGACUC UAGUCUGGCA CGGUG AAGA GACAUGAGAG GUGUAGAAUA AGUGGGAGGC CCCCGGCGCC CCCCCGUUCC CCGCGAGGGG UCGGGGCGGG GUCCGC CGG CCCUGCGGGC CGCCGGUGAA AUACCACUAC UCUCAUCGUU UUUUCACUGA CCCGGUGAGG CGGGGGGGCG AGCCCCG AG GGGCUCUCGC UUCUGGCGCC AAGCGCCCGU CCGCGCGCGC GGGCGGGCGC GACCCGCUCC GGGGACAGUG CCAGGUGG G GAGUUUGACU GGGGCGGUAC ACCUGUCAAA CGGUAACGCA GGUGUCCUAA GGCGAGCUCA GGGAGGACAG AAACCUCCC GUGGAGCAGA AGGGCAAAAG CUCGCUUGAU CUUGAUUUUC AGUACGAAUA CAGACCGUGA AAGCGGGGCC UCACGAUCCU UCUGACCUU UUGGGUUUUA AGCAGGAGGU GUCAGAAAAG UUACCACAGG GAUAACUGGC UUGUGGCGGC CAAGCGUUCA U AGCGACGU CGCUUUUUGA UCCUUCGAUG UCGGCUCUUC CUAUCAUUGU GAAGCAGAAU UCACCAAGCG UUGGAUUGUU CA CCCACUA AUAGGGAACG UGAGCUGGGU UUAGACCGUC GUGAGACAGG UUAGUUUUAC CCUACUGAUG AUGUGUUGUU GCC AUGGUA AUCCUGCUCA GUACGAGAGG AACCGCAGGU UCAGACAUUU GGUGUAUGUG CUUGGCUGAG GAGCCAAUGG GGCG AAGCU ACCAUCUGUG GGAUUAUGAC UGAACGCCUC UAAGUCAGAA UCCCGCCCAG GCGGAACGAU ACGGCAGCGC CGAAG GAGC CUCGGUUGGC CCCGGAUAGC CGGCUCCCCG UCCGUCCCCG UCCGGCGGGU CCCCGCCUCG UCGCCCCCCC GGGUGC GGG GCGGGUCCCC CCGCCGGGCG UCGGGACCGG GGUCCGGUGC GGAGAGCCAU UCGUCCCGGG AAACGGGGUG CGGCCGG AA AGGGGGCCGC CCUCUCGCCC GUCACGCUUA ACGCACGUUC GUGUGGAACC UGGCGCUAAA CCAUUCGUAG ACGACCUG C UUCUGGGUCG GGGUUUCGUA CGUAGCAGAG CAGCUCCCUC GCUGCGAUCU AUUGAAAGUC AGCCCUCGAC ACAAGGGUU UGU

Macromolecule #80: RNA (76-MER)

• Macromolecule: Name: RNA (76-MER) / type: rna / ID: 80 ; Details: A/P and P/E tRNAs. For some reason it has united the two tRNAs into one molecule, although these are different molecules with different sequences. As the structure contains heterogeneous population of tRNAs then the identity of tRNA is unknown.; Number of copies: 1
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 24.414451 KDa

Sequence

String:

GCCCGGAUAG CUCAGUCGGU AGAGCAGGGG AUUCCGUAUC CCCGUGUCCU UGGUUCGAUU CCGAGUCCGG GCACCA

GENBANK: GENBANK: CP054379.1

Macromolecule #81: RNA (76-MER)

• Macromolecule: Name: RNA (76-MER) / type: rna / ID: 81 ; Details: A/P and P/E tRNAs. For some reason it has united the two tRNAs into one molecule, although these are different molecules with different sequences.As the structure contains heterogeneous population of tRNAs then the identity of tRNA is unknown.; Number of copies: 1
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 24.436508 KDa

Sequence

String:

UGCCCGGUAG CCCCGAAGGU AGAGCAGGGG AUUCCGAAUC CCCGUGUCCU UGGUUCGAUU CCGAGUCCGG GCACCA

GENBANK: GENBANK: CP056235.1

Macromolecule #82: RNA (5'-D(P*()P*()P*()P*())-R(P*UP*UP*AP*CP*GP*GP*CP*GP*GP*UP*()P...

• Macromolecule: Name: RNA (5'-D(P*()P*()P*()P*())-R(P*UP*UP*AP*CP*GP*GP*CP*GP*GP*UP*()P*()P*()P*()P*()P*())-3'); type: rna / ID: 82 / Number of copies: 1
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 6.3608 KDa

Sequence

String:

UCAUCUACGG CGGUAGAUCU

Macromolecule #2: 40S ribosomal protein SA

• Macromolecule: Name: 40S ribosomal protein SA / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 32.853914 KDa

Sequence

String:

MSGGLDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQAAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA HSVGLMWWML AREVLRMRGT ISREHPWEVM PDLYFYRDPE EIEKEEQAAA EKAVTKEEFQ GEWTAPAPEF TA AQPEVAD WSEGVQVPSV PIQQFPTEDW SAQPATEDWS AAPTAQATEW VGATTEWS

UniProtKB: Small ribosomal subunit protein uS2

Macromolecule #3: 40S ribosomal protein S3a

• Macromolecule: Name: 40S ribosomal protein S3a / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 30.002061 KDa

Sequence

String:

MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM MEIMTREVQT NDLKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHGEGGSS GK TTGDETG AKVERADGYE PPVQESV

UniProtKB: Small ribosomal subunit protein eS1

Macromolecule #4: 40S ribosomal protein S3

• Macromolecule: Name: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-(apurinic or apyrimidinic site) lyase
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 26.715344 KDa

Sequence

String:

MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAE KVATRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFIMES GAKGCEVVVS GKLRGQRAKS MKFVDGLMIH S GDPVNYYV DTAVRHVLLR QGVLGIKVKI MLPWDPSGKI GPKKPLPDHV SIVEPKDEIL PTTPISEQKG GKPEPPAMPQ PV PTA

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #5: 40S ribosomal protein S4, X isoform

• Macromolecule: Name: 40S ribosomal protein S4, X isoform / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 29.654869 KDa

Sequence

String:

MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE VKKICMQRFI KIDGKVRTDI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ITDFIKFDTG NLCMVTGGAN LGRIGVITNR ERHPGSFDVV HVKDANGNSF ATRLSNIFVI GKGNKPWISL PR GKGIRLT IAEERDKRLA AKQSSG

UniProtKB: Small ribosomal subunit protein eS4

Macromolecule #6: 40S ribosomal protein S5

• Macromolecule: Name: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 22.94348 KDa

Sequence

String:

MTEWETATPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT IAECLADELI NAAKGSSNSY AIKKKDELER VAKSNR

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #7: 40S ribosomal protein S7

• Macromolecule: Name: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 22.168914 KDa

Sequence

String:

MFSSSAKIVK PNGEKPDEFE SGISQALLEL EMNSDLKAQL RELNITAAKE IEVGGGRKAI IIFVPVPQLK SFQKIQVRLV RELEKKFSG KHVVFIAQRR ILPKPTRKSR TKNKQKRPRS RTLTAVHDAI LEDLVFPSEI VGKRIRVKLD GSRLIKVHLD K AQQNNVEH KVETFSGVYK KLTGKDVNFE FPEFQL

UniProtKB: Small ribosomal subunit protein eS7

Macromolecule #8: 40S ribosomal protein S8

• Macromolecule: Name: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 24.263387 KDa

Sequence

String:

MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ GKLLACIASR PGQCGRADGY VLEGKELEFY LRKIKARKGK

UniProtKB: Small ribosomal subunit protein eS8

Macromolecule #9: 40S ribosomal protein S10

• Macromolecule: Name: 40S ribosomal protein S10 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 18.951818 KDa

Sequence

String:

MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSRP ETGRPRPKGP EGERPARFTR GEADRDTYRR SAVPPGADKK AEAGAGSATE FQFRGGFGRG R GQPPQ

UniProtKB: Small ribosomal subunit protein eS10

Macromolecule #10: 40S ribosomal protein S11

• Macromolecule: Name: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 18.468826 KDa

Sequence

String:

MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #11: 40S ribosomal protein S15

• Macromolecule: Name: 40S ribosomal protein S15 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 17.076207 KDa

Sequence

String:

MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLNRGLR RKQHSLLKRL RKAKKEAPPM EKPEVVKTHL RDMIILPEM VGSMVGVYNG KTFNQVEIKP EMIGHYLGEF SITYKPVKHG RPGIGATHSS RFIPLK

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #12: Rps16 protein

• Macromolecule: Name: Rps16 protein / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 16.477377 KDa

Sequence

String:

MPSKGPLQSV QVFGRKKTAT AVAHCKRGNG LIKVNGRPLE MIEPRTLQYK LLEPVLLLGK ERFAGVDIRV RVKGGGHVAQ IYAIRQSIS KALVAYYQKY VDEASKKEIK DILIQYDRTL LVADPRRCES KKFGGPGARA RYQKSYR

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #13: 40S ribosomal protein S17

• Macromolecule: Name: 40S ribosomal protein S17 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 15.537043 KDa

Sequence

String:

MGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKNLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERER RDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGMNFK TPRGAV

UniProtKB: Small ribosomal subunit protein eS17

Macromolecule #14: 40S ribosomal protein S18

• Macromolecule: Name: 40S ribosomal protein S18 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 17.759777 KDa

Sequence

String:

MSLVIPEKFQ HILRVLNTNI DGRRKIAFAI TAIKGVGRRY AHVVLRKADI DLTKRAGELT EDEVERVITI MQNPRQYKIP DWFLNRQKD VKDGKYSQVL ANGLDNKLRE DLERLKKIRA HRGLRHFWGL RVRGQHTKTT GRRGRTVGVS KKK

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #15: 40S ribosomal protein S19

• Macromolecule: Name: 40S ribosomal protein S19 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 16.11756 KDa

Sequence

String:

MPGVTVKDVN QQEFVRALAA FLKKSGKLKV PEWVDTVKLA KHKELAPYDE NWFYTRAAST ARHLYLRGGA GVGSMTKIYG GRQRNGVRP SHFSRGSKSV ARRVLQALEG LKMVEKDQDG GRKLTPQGQR DLDRIAGQVA AANKKH

UniProtKB: Small ribosomal subunit protein eS19

Macromolecule #16: 40S ribosomal protein S20

• Macromolecule: Name: 40S ribosomal protein S20 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 13.398763 KDa

Sequence

String:

MAFKDTGKTP VEPEVAIHRI RITLTSRNVK SLEKVCADLI RGAKEKNLKV KGPVRMPTKT LRITTRKTPC GEGSKTWDRF QMRIHKRLI DLHSPSEIVK QITSISIEPG VEVEVTIADA

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #17: 40S ribosomal protein S21

• Macromolecule: Name: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 9.140351 KDa

Sequence

String:

MQNDAGEFVD LYVPRKCSAS NRIIAAKDHA SIQMNVAEVD RSTGRFNGQF KTYGICGAIR RMGESDDSIL RLAKADGIVS KNF

UniProtKB: Small ribosomal subunit protein eS21

Macromolecule #18: 40S ribosomal protein S23

• Macromolecule: Name: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 15.844666 KDa

Sequence

String:

MGKCRGLRTA RKLRSHRRDQ KWHDKQYKKA HLGTALKANP FGGASHAKGI VLEKVGVEAK QPNSAIRKCV RVQLIKNGKK ITAFVPNDG CLNFIEENDE VLVAGFGRKG HAVGDIPGVR FKVVKVANVS LLALYKGKKE RPRS

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #19: 40S ribosomal protein S26

• Macromolecule: Name: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 13.047532 KDa

Sequence

String:

MTKKRRNNGR AKKGRGHVQP IRCTNCARCV PKDKAIKKFV IRNIVEAAAV RDISEASVFD AYVLPKLYVK LHYCVSCAIH SKVVRNRSR EARKDRTPPP RFRPAGAAPR PPPKPM

UniProtKB: 40S ribosomal protein S26

Macromolecule #20: 40S ribosomal protein S28

• Macromolecule: Name: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 7.855052 KDa

Sequence

String:

MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE SEREARRLR

UniProtKB: Small ribosomal subunit protein eS28

Macromolecule #21: 40S ribosomal protein S29

• Macromolecule: Name: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 6.690821 KDa

Sequence

String:

MGHQQLYWSH PRKFGQGSRS CRVCSNRHGL IRKYGLNMCR QCFRQYAKDI GFIKLD

UniProtKB: Small ribosomal subunit protein uS14

Macromolecule #22: Receptor of activated protein C kinase 1

• Macromolecule: Name: Receptor of activated protein C kinase 1 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 35.115652 KDa

Sequence

String:

MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC GWDKLVKVWN LANCKLKTNH IGHTGYLNTV TVSPDGSLCA SGGKDGQAML WDLNEGKHLY TLDGGDIINA LC FSPNRYW LCAATGPSIK IWDLEGKIIV DELKQEVIST SSKAEPPQCT SLAWSADGQT LFAGYTDNLV RVWQVTIGTR

UniProtKB: Small ribosomal subunit protein RACK1

Macromolecule #23: 40S ribosomal protein S2

• Macromolecule: Name: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 31.283387 KDa

Sequence

String:

MADDAGAAGG PGGPGGPGLG GRGGFRGGFG SGLRGRGRGR GRGRGRGRGA RGGKAEDKEW IPVTKLGRLV KDMKIKSLEE IYLFSLPIK ESEIIDFFLG ASLKDEVLKI MPVQKQTRAG QRTRFKAFVA IGDYNGHVGL GVKCSKEVAT AIRGAIILAK L SIVPVRRG YWGNKIGKPH TVPCKVTGRC GSVLVRLIPA PRGTGIVSAP VPKKLLMMAG IDDCYTSARG CTATLGNFAK AT FDAISKT YSYLTPDLWK ETVFTKSPYQ EFTDHLVKTH TRVSVQRTQA PAVATT

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #24: 40S ribosomal protein S6

• Macromolecule: Name: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 28.751906 KDa

Sequence

String:

MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRRRLSSLR AS TSKSESS QK

UniProtKB: Small ribosomal subunit protein eS6

Macromolecule #25: 40S ribosomal protein S9

• Macromolecule: Name: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 22.641564 KDa

Sequence

String:

MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG RPGRVKRKNA KKGQGGAGAG DDEEED

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #26: 40S ribosomal protein S12

• Macromolecule: Name: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 14.549001 KDa

Sequence

String:

MAEEGIAAGG VMDVNTALQE VLKTALIHDG LARGIREAAK ALDKRQAHLC VLASNCDEPM YVKLVEALLA EHQINLIKVD DNKKLGEWV GLCKIDREGK PRKVVGCSCV VVKDYGKESQ AKDVIEEYFK CKK

UniProtKB: Small ribosomal subunit protein eS12

Macromolecule #27: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 17.259389 KDa

Sequence

String:

MGRMHAPGKG LSQSALPYRR SVPTWLKLTS DDVKEQIYKL AKKGLTPSQI GVILRDSHGV AQVRFVTGNK ILRILKSKGL APDLPEDLY HLIKKAVAVR KHLERNRKDK DAKFRLILIE SRIHRLARYY KTKRVLPPNW KYESSTASAL VA

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #28: 40S ribosomal protein S14

• Macromolecule: Name: 40S ribosomal protein S14 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 16.288745 KDa

Sequence

String:

MAPRKGKDKK EEQVISLGPQ VAEGENVFGV CHIFASFNDT FVHVTDLSGK ETICRVTGGM KVKADRDESS PYAAMLAAQD VAQRCKELG ITALHIKLRA TGGNRTKTPG PGAQSALRAL ARSGMKIGRI EDVTPIPSDS TRRKGGRRGR RL

UniProtKB: Small ribosomal subunit protein uS11

Macromolecule #29: 40S ribosomal protein S15a

• Macromolecule: Name: 40S ribosomal protein S15a / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 14.865555 KDa

Sequence

String:

MVRMNVLADA LKSINNAEKR GKRQVLIRPC SKVIVRFLTV MMKHGYIGEF EIIDDHRAGK IVVNLTGRLN KCGVISPRFD VQLKDLEKW QNNLLPSRQF GFIVLTTSAG IMDHEEARRK HTGGKILGFF F

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #30: 40S ribosomal protein S24

• Macromolecule: Name: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 15.463333 KDa

Sequence

String:

MNDTVTIRTR KFMTNRLLQR KQMVIDVLHP GKATVPKTEI REKLAKMYKT TPDVIFVFGF RTHFGGGKTT GFGMIYDSLD YAKKNEPKH RLARHGLYEK KKTSRKQRKE RKNRMKKVRG TAKANVGAGK KPKE

UniProtKB: Small ribosomal subunit protein eS24

Macromolecule #31: 40S ribosomal protein S25

• Macromolecule: Name: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 13.776224 KDa

Sequence

String:

MPPKDDKKKK DAGKSAKKDK DPVNKSGGKA KKKKWSKGKV RDKLNNLVLF DKATYDKLCK EVPNYKLITP AVVSERLKIR GSLARAALQ ELLSKGLIKL VSKHRAQVIY TRNTKGGDAP AAGEDA

UniProtKB: 40S ribosomal protein S25

Macromolecule #32: 40S ribosomal protein S27

• Macromolecule: Name: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 9.480186 KDa

Sequence

String:

MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

UniProtKB: 40S ribosomal protein S27

Macromolecule #33: Ubiquitin-like domain-containing protein

• Macromolecule: Name: Ubiquitin-like domain-containing protein / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 6.668938 KDa

Sequence

String:

KVHGSLARAG KVRGQTPKVA KQEKKKKKTG RAKRRMQYNR RFVNVVPTFG KKKGPNANS

UniProtKB: Ubiquitin-like domain-containing protein

Macromolecule #34: Ubiquitin-40S ribosomal protein S27a

• Macromolecule: Name: Ubiquitin-40S ribosomal protein S27a / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 17.990014 KDa

Sequence

String:

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGAKKR KKKSYTTPK KNKHKRKKVK LAVLKYYKVD ENGKISRLRR ECPSDECGAG VFMGSHFDRH YCGKCCLTYC FNKPEDK

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

Macromolecule #35: 60S ribosomal protein L8

• Macromolecule: Name: 60S ribosomal protein L8 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 28.088863 KDa

Sequence

String:

MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVY CGKKAQLNIG NVLPVGTMPE GTIVCCLEEK PGDRGKLARA SGNYATVISH NPETKKTRVK LPSGSKKVIS S ANRAVVGV VAGGGRIDKP ILKAGRAYHK YKAKRNCWPR VRGVAMNPVE HPFGGGNHQH IGKPSTIRRD APAGRKVGLI AA RRTGRLR GTKTVQEKEN

UniProtKB: Large ribosomal subunit protein uL2

Macromolecule #36: 60S ribosomal protein L3

• Macromolecule: Name: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 46.23818 KDa

Sequence

String:

MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD DTGKKQLEKD FNSMKKYCQV I RIIAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVN QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKTHRG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINPL GGF VHYGEV TNDFIMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTMEEKK AFMGPLKKDR IAKE EGA

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #37: 60S ribosomal protein L4

• Macromolecule: Name: 60S ribosomal protein L4 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 47.363113 KDa

Sequence

String:

MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHCVEEVPE LPLVVEDKVE S YKKTKEAV QLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC IIYNEDNGII KAFRNIPGIT LLNVSKLNIL KL APGGHVG RFCIWTESAF RKLDELYGTW RKAASLKSNY NLPMHKMMNT DLSRILKSPE IQRALRAPRK KIHRRVLKKN PLK NLRIML KLNPYAKTMR RNTILRQARN HKLRVKKLEA AAAALAAKSE KIVPEKGAGD KKPAVGKKGK KPVDAKKLKK PAGK KVVTK KPAEKKPTTE EKKSAA

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #40: 60S ribosomal protein L5

• Macromolecule: Name: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 34.52284 KDa

Sequence

String:

MGFVKVVKNK AYFKRYQVRF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVNGDE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD GGLSIPHSTK RFPGYDSESK EFNAEVHRKH IMGQNVADYM RYLMEEDEDA YKKQFSQYIK NNVTPDMMEE MY KKAHAAI RENPVYEKKP KREVKKKRWN RPKMSLAQKK DRVAQKKASF LRAQERAAES

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #41: 60S ribosomal protein L6

• Macromolecule: Name: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 33.64491 KDa

Sequence

String:

MAGEKAEKPD KKEQKPAAKK AGGDATAPRA LPAGAVKKSS SKAKKLRKSK PHCSRNPVLV RGIGRYSRSA MYSRKALYKR KYSAAKTKV EKKKKKEKVL ATVTKTVGGD KNGGTRVVKL RKMPRYYPTE DVPRKLLSHG KKPFSQHVRR LRSSITPGTV L IILTGRHR GKRVVFLKQL GSGLLLVTGP LALNRVPLRR THQKFVIATS TKVDISKVKI PKHLTDAYFK KKPLRKPRHQ EG EIFDTEK EKYEITEQRK ADQKAVDSQI LPKIKAVPQL QGYLRSQFSL TNGMYPHKLV F

UniProtKB: Large ribosomal subunit protein eL6

Macromolecule #42: 60S ribosomal protein L7

• Macromolecule: Name: 60S ribosomal protein L7 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 30.39948 KDa

Sequence

String:

MEAVPEKKKK VAAALGTLKK KKVPAVPETL KKKRRNFAEL KVKRLRKKFA LKTLRKARRK LIYEKAKHYH KEYRQMYRTE IRMARMARK AGNFYVPAEP KLAFVIRIRG INGVSPKVRK VLQLLRLRQI FNGTFVKLNK ASVNMLRIVE PYIAWGYPNL K SVNELIYK RGYGKINKKR IALTDNSLVA RSLGKFGIIC MEDLIHEIYT VGKRFKEANN FLWPFKLSSP RGGMKKKTTH FV EGGDAGN REDQINRLIR RMN

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #43: 60S ribosomal protein L7a

• Macromolecule: Name: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 30.061785 KDa

Sequence

String:

MPKGKKAKGK KVAPAPAVVK KQEAKKVVNP LFEKRPKNFG IGQDIQPKRD LTRFVKWPRY IRLQRQRAIL YKRLKVPPAI NQFTQALDR QTATQLLKLA HKYRPETKQE KKQRLLARAE KKAAGKGDVP TKRPPVLRAG VNTVTTLVEN KKAQLVVIAH D VDPIELVV FLPALCRKMG VPYCIIKGKA RLGRLVHRKT CTTVAFTQVN SEDKGALAKL VEAIRTNYND RYDEIRRHWG GN VLGPKSV ARIAKLEKAK AKELATKLG

UniProtKB: Large ribosomal subunit protein eL8

Macromolecule #44: 60S ribosomal protein L9

• Macromolecule: Name: 60S ribosomal protein L9 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 21.929496 KDa

Sequence

String:

MKTILSNQTV DIPENVDITL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR TGVACSVSQA QKDELILEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QPDE

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #45: 60S ribosomal protein L10

• Macromolecule: Name: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 24.657084 KDa

Sequence

String:

MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLCG HMVSDEYEQL SSEALEAARI CANKYMVKSC GKDGFHIRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNKEHVI EALRRAKFKF P GRQKIHIS KKWGFTKFNA DEFEDMVAEK RLIPDGCGVK YIPNRGPLDK WRALHS

UniProtKB: Large ribosomal subunit protein uL16

Macromolecule #46: 60S ribosomal protein L11

• Macromolecule: Name: 60S ribosomal protein L11 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 20.288465 KDa

Sequence

String:

MAQDQGEKEN PMRELRIRKL CLNICVGESG DRLTRAAKVL EQLTGQTPVF SKARYTVRSF GIRRNEKIAV HCTVRGAKAE EILEKGLKV REYELRKNNF SDTGNFGFGI QEHIDLGIKY DPSIGIYGLD FYVVLGRPGF SIADKKRRTG CIGAKHRISK E EAMRWFQQ KYDGIILPGK

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #47: 60S ribosomal protein L13

• Macromolecule: Name: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 24.368693 KDa

Sequence

String:

MAPSRNGMIL KPHFHKDWQQ RVDTWFNQPA RKIRRRKARQ AKARRIAPRP ASGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK MARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLILFPR KPSAPKKGDS SAEELKLATQ LTGPVMPIRN V YKKEKARA ITEEEKNFKA FASLRMARAN ARLFGIRAKR AKEAAEQDVE KKK

UniProtKB: Large ribosomal subunit protein eL13

Macromolecule #48: 60S ribosomal protein L14

• Macromolecule: Name: 60S ribosomal protein L14 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 23.393998 KDa

Sequence

String:

MVFRRFVEVG RVAYISFGPH AGKLVAIVDV IDQNRALVDG PCTRVRRQAM PFKCMQLTDF ILKFPHSARQ KYVRKAWEKA DINTKWAAT RWAKKIDARE RKAKMTDFDR FKVMKAKKMR NRIIKTEVKK LQRAALLKAS PKKAAVAKAA IAAAAAAKAK V PAKKATGP GQKAAAQKAS AQKAAGQKAA PPAKGQKGQK TPAQKAPAPK AAGKKA

UniProtKB: Large ribosomal subunit protein eL14

Macromolecule #49: Ribosomal protein L15

• Macromolecule: Name: Ribosomal protein L15 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 24.207285 KDa

Sequence

String:

MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG RKSRGLGKGH KFHHTIGGSR RAAWRRRNTL QLHRYR

UniProtKB: Ribosomal protein L15

Macromolecule #50: 60S ribosomal protein L13a

• Macromolecule: Name: 60S ribosomal protein L13a / type: protein_or_peptide / ID: 50 ; Details: Please keep residue Gly 13 as it is. The Mass-spectrometry data shows that leading razor peptide for this protein is Uniprot Q5RK10.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 23.501316 KDa

Sequence

String:

MAEGQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL ERLKVLDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY RKKKQLLRLR KQAEKNVEKK ICKFTEVLKT NGLLV

UniProtKB: Large ribosomal subunit protein uL13

Macromolecule #51: 60S ribosomal protein L17

• Macromolecule: Name: 60S ribosomal protein L17 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 21.444221 KDa

Sequence

String:

MVRYSLDPEN PTKSCKSRGS NLRVHFKNTR ETAQAIKGMH IRKATKYLKD VTLKKQCVPF RRYNGGVGRC AQAKQWGWTQ GRWPKKSAE FLLHMLKNAE SNAELKGLDV DSLVIEHIQV NKAPKMRRRT YRAHGRINPY MSSPCHIEMI LTEKEQIVPK P EEEVAQKK KISQKKLKKQ KLMARE

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #52: 60S ribosomal protein L18

• Macromolecule: Name: 60S ribosomal protein L18 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 21.712768 KDa

Sequence

String:

MGVDIRHNKD RKVRRKEPKS QDIYLRLLVK LYRFLARRTN STFNQVVLKR LFMSRTNRPP LSLSRMIRKM KLPGRENKTA VVVGTITDD VRILEVPKLK VCALRVSSRA RSRILKAGGK ILTFDQLALE SPKGRGTVLL SGPRKGREVY RHFGKAPGTP H SHTKPYVR SKGRKFERAR GRRASRGYKN

UniProtKB: Large ribosomal subunit protein eL18

Macromolecule #53: Ribosomal protein L19

• Macromolecule: Name: Ribosomal protein L19 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 23.535281 KDa

Sequence

String:

MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ARKRREERLQ AKKEEIIKTL SKEEETKK

UniProtKB: Ribosomal protein L19

Macromolecule #54: 60S ribosomal protein L18a

• Macromolecule: Name: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 20.778486 KDa

Sequence

String:

MKASGTLREY KVVGRCLPTP KCHTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAAGKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTFF

UniProtKB: Large ribosomal subunit protein eL20

Macromolecule #55: 60S ribosomal protein L21

• Macromolecule: Name: 60S ribosomal protein L21 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 18.624014 KDa

Sequence

String:

MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIIVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

UniProtKB: 60S ribosomal protein L21

Macromolecule #56: 60S ribosomal protein L22

• Macromolecule: Name: 60S ribosomal protein L22 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 14.784962 KDa

Sequence

String:

MAPVKKLVAK GGKKKKQVLK FTLDCTHPVE DGIMDAANFE QFLQERIKVN GKAGNLGGGV VTIERSKSKI TVTSEVPFSK RYLKYLTKK YLKKNNLRDW LRVVANSKES YELRYFQINQ DEEEEEDED

UniProtKB: Large ribosomal subunit protein eL22

Macromolecule #57: 60S ribosomal protein L23

• Macromolecule: Name: 60S ribosomal protein L23 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 14.892505 KDa

Sequence

String:

MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYR RKDGVFLYFE DNAGVIVNNK GEMKGSAITG PVAKECADLW PRIASNAGSI A

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #58: 60S ribosomal protein L24

• Macromolecule: Name: 60S ribosomal protein L24 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 17.825111 KDa

Sequence

String:

MKVELCSFSG YKIYPGHGRR YARTDGKVFQ FLNAKCESAF LSKRNPRQIN WTVLYRRKHK KGQSEEIQKK RTRRAVKFQR AITGASLAD IMAKRNQKPE VRKAQREQAI RAAKEAKKAK QASKKTAMAA AKAPTKAAPK QKIVKPVKVS APRVGGKR

UniProtKB: Large ribosomal subunit protein eL24

Macromolecule #59: 60S ribosomal protein L23a

• Macromolecule: Name: 60S ribosomal protein L23a / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 17.740193 KDa

Sequence

String:

MAPKAKKEAP APPKAEAKAK ALKAKKAVLK GVHSHKKKKI RTSPTFRRPK TLRLRRQPKY PRKSAPRRNK LDHYAIIKFP LTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #60: 60S ribosomal protein L26

• Macromolecule: Name: 60S ribosomal protein L26 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 17.32241 KDa

Sequence

String:

MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI RPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGKYKEET IEKMQE

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #61: 60S ribosomal protein L27

• Macromolecule: Name: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 15.835831 KDa

Sequence

String:

MGKFMKPGKV VLVLAGRYSG RKAVIVKNID DGTSDRPYSH ALVAGIDRYP RKVTAAMGKK KIAKRSKIKS FVKVYNYNHL MPTRYSVDI PLDKTVVNKD VFRDPALKRK ARREAKVKFE ERYKTGKNKW FFQKLRF

UniProtKB: 60S ribosomal protein L27

Macromolecule #62: 60S ribosomal protein L27a

• Macromolecule: Name: 60S ribosomal protein L27a / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 16.661613 KDa

Sequence

String:

MPSRLRKTRK LRGHVSHGHG RIGKHRKHPG GRGNAGGMHH HRINFDKYHP GYFGKVGMRH YHLKRNQSFC PTVNLDKLWT LVSEQTRVN AAKNKNGVAP IIDVVRSGYY KVLGKGKLPK QPVIVKAKFF SRRAEEKIKG VGGACVLVA

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #63: 60S ribosomal protein L29

• Macromolecule: Name: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 17.374844 KDa

Sequence

String:

MAKSKNHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAVSARAE AIKALVKPQA VKPKMPKGS SRKLSRLAFI AHPKLGKKIR SYMAKGRRLC QPKPKVQTKA EAKAPAKAQA KAPAQAPKGA QAPVKAP

UniProtKB: Large ribosomal subunit protein eL29

Macromolecule #64: 60S ribosomal protein L30

• Macromolecule: Name: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 12.805092 KDa

Sequence

String:

MVAAKKTKKS LESINSRLQL VMKSGKYVLG YKQTLKMIRQ GKAKLVILAN NCPALRKSEI EYYAMLAKTG VHHYSGNNIE LGTACGKYY RVCTLAIIDP GDSDIIRSMP EQTGEK

UniProtKB: Large ribosomal subunit protein eL30

Macromolecule #65: 60S ribosomal protein L31

• Macromolecule: Name: 60S ribosomal protein L31 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 14.494938 KDa

Sequence

String:

MAPAKKGGEK KKGRSAINEV VTREYTINIH KRIHGVGFKK RAPRALKEIR KFAMKEMGTP DVRIDTRLNK AVWAKGIRNV PYRIRVRLS RKRNEDEDSP NKLYTLVTYV PVTTFKNLQT VNVDEN

UniProtKB: Large ribosomal subunit protein eL31

Macromolecule #66: 60S ribosomal protein L32

• Macromolecule: Name: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 15.898932 KDa

Sequence

String:

MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLL MCNKSYCAEI AHNVSSKNRK AIVERAAQLA IRVTNPNARL RSEENE

UniProtKB: Large ribosomal subunit protein eL32

Macromolecule #67: 60S ribosomal protein L35a

• Macromolecule: Name: 60S ribosomal protein L35a / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 12.580809 KDa

Sequence

String:

MSGRLWCKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

UniProtKB: Large ribosomal subunit protein eL33

Macromolecule #68: 60S ribosomal protein L34

• Macromolecule: Name: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 13.326074 KDa

Sequence

String:

MVQRLTYRRR LSYNTASNKT RLSRTPGNRI VYLYTKKVGK APKSACGVCP GRLRGVRAVR PKVLMRLSKT KKHVSRAYGG SMCAKCVRD RIKRAFLIEE QKIVVKVLKA QAQSQKAK

UniProtKB: Large ribosomal subunit protein eL34

Macromolecule #69: 60S ribosomal protein L35

• Macromolecule: Name: 60S ribosomal protein L35 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 14.595702 KDa

Sequence

String:

MAKIKARDLR GKKKEELLKQ LDDLKVELSQ LRVAKVTGGA ASKLSKIRVV RKSIARVLTV INQTQKENLR KFYKGKKYKP LDLRPKKTR AMRRRLTKHE EKLKTKKQQR KERLYPLRKY AVKA

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #70: 60S ribosomal protein L36

• Macromolecule: Name: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 70 ; Details: Please keep the Gly 49 as it is. Mass-spectrometry data indicates that leading razor peptide is D4A1Q0.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 12.290859 KDa

Sequence

String:

MALRYPMAVG LNKGHKVTKN VSKPRHSRRR GRLTKHTKFV RDMIREVCGF APYERRAMEL LKVSKDKRAL KFIKKRVGTH IRAKRKREE LSNVLAAMRK AAAKKD

UniProtKB: Large ribosomal subunit protein eL36

Macromolecule #71: Ribosomal protein L37

• Macromolecule: Name: Ribosomal protein L37 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 11.111032 KDa

Sequence

String:

MTKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRA AVAASSSS

UniProtKB: Ribosomal protein L37

Macromolecule #72: 60S ribosomal protein L38

• Macromolecule: Name: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 8.238948 KDa

Sequence

String:

MPRKIEEIKD FLLTARRKDA KSVKIKKNKD NVKFKVRCSR YLYTLVITDK EKAEKLKQSL PPGLAVKELK

UniProtKB: Large ribosomal subunit protein eL38

Macromolecule #73: 60S ribosomal protein L39

• Macromolecule: Name: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 6.426759 KDa

Sequence

String:

MSSHKTFRIK RFLAKKQKQN RPIPQWIRMK TGNKIRYNSK RRHWRRTKLG L

UniProtKB: Large ribosomal subunit protein eL39

Macromolecule #74: Ubiquitin-60S ribosomal protein L40

• Macromolecule: Name: Ubiquitin-60S ribosomal protein L40 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 14.758394 KDa

Sequence

String:

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGIIEP SLRQLAQKY NCDKMICRKC YARLHPRAVN CRKKKCGHTN NLRPKKKVK

UniProtKB: Ubiquitin-ribosomal protein eL40 fusion protein

Macromolecule #75: 60S ribosomal protein L41

• Macromolecule: Name: 60S ribosomal protein L41 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 3.473451 KDa

Sequence

String:

MRAKWRKKRM RRLKRKRRKM RQRSK

UniProtKB: Small ribosomal subunit protein eS32

Macromolecule #76: Ribosomal protein L36a

• Macromolecule: Name: Ribosomal protein L36a / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 12.476973 KDa

Sequence

String:

MVNVPKTRRT FCKKCGKHQP HKVTQYKKGK DSLYAQGKRR YDRKQSGYGG QTKPIFRKKA KTTKKIVLRL ECVEPNCRSK RMLAIKRCK HFELGGDKKR KGQVIQF

UniProtKB: Ribosomal protein L36a

Macromolecule #77: 60S ribosomal protein L37a

• Macromolecule: Name: 60S ribosomal protein L37a / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 10.299283 KDa

Sequence

String:

MAKRTKKVGI VGKYGTRYGA SLRKMVKKIE ISQHAKYTCS FCGKTKMKRR AVGIWHCGSC MKTVAGGAWT YNTTSAVTVK SAIRRLEEL KDQ

UniProtKB: Large ribosomal subunit protein eL43

Macromolecule #78: 60S ribosomal protein L28

• Macromolecule: Name: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 15.770597 KDa

Sequence

String:

MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVMKRRSGQ RKPATSYVRT TINKNARAT LSSIRHMIRK NKYRPDLRMA AIRRASAILR SQKPVVVKRK RTRPTKSS

UniProtKB: Large ribosomal subunit protein eL28

Macromolecule #83: 60S ribosomal protein L10a

• Macromolecule: Name: 60S ribosomal protein L10a / type: protein_or_peptide / ID: 83 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 24.879422 KDa

Sequence

String:

MSSKVSRDTL YEAVREVLHG NQRKRRKFLE TVELQISLKN YDPQKDKRFS GTVRLKSTPR PKFSVCVLGD QQHCDEAKAV DIPHMDIEA LKKLNKNKKL VKKLAKKYDA FLASESLIKQ IPRILGPGLN KAGKFPSLLT HNENMVAKVD EVKSTIKFQM K KVLCLAVA VGHVKMTDDE LVYNIHLAVN FLVSLLKKNW QNVRALYIKS TMGKPQRLY

UniProtKB: Large ribosomal subunit protein uL1

Macromolecule #84: ALA-ALA-LYS-ALA

• Macromolecule: Name: ALA-ALA-LYS-ALA / type: protein_or_peptide / ID: 84 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rattus norvegicus (Norway rat)
• Molecular weight: Theoretical: 360.428 Da

Sequence

String:

AAKA

Macromolecule #85: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 85 / Number of copies: 33 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #86: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 86 / Number of copies: 6 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #87: water

• Macromolecule: Name: water / type: ligand / ID: 87 / Number of copies: 8 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

Buffer

pH: 7.5
Component:

Concentration	Formula	Name
20.0 mM	HEPES	4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
150.0 mM	KOAc	potassium acetate
4.0 mM	MgOAc	magnesium acetate
0.5 mM	TCEP	tris(2-carboxyethyl)phosphine

• Grid: Model: C-flat-1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 3 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 10 sec.
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 10 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Number grids imaged: 1 / Number real images: 3644 / Average electron dose: 30.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 70.0 µm / Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 59000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 499054

Startup model

Type of model: EMDB MAP
EMDB ID:

0600

• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.86 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 62369
• Initial angle assignment: Type: ANGULAR RECONSTITUTION / Software - Name: RELION
• Final angle assignment: Type: ANGULAR RECONSTITUTION / Software - Name: RELION
• Final 3D classification: Software - Name: RELION

Atomic model buiding 1

Initial model

PDB ID:

6ole

Chain - Source name: PDB / Chain - Initial model type: experimental model

• Refinement: Space: REAL / Protocol: RIGID BODY FIT

Output model

PDB-7qgg:
Neuronal RNA granules are ribosome complexes stalled at the pre-translocation state