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TitleCiliary central apparatus structure reveals mechanisms of microtubule patterning.
Journal, issue, pagesNat Struct Mol Biol, Vol. 29, Issue 5, Page 483-492, Year 2022
Publish dateMay 16, 2022
AuthorsMiao Gui / Xiangli Wang / Susan K Dutcher / Alan Brown / Rui Zhang /
PubMed AbstractA pair of extensively modified microtubules form the central apparatus (CA) of the axoneme of most motile cilia, where they regulate ciliary motility. The external surfaces of both CA microtubules ...A pair of extensively modified microtubules form the central apparatus (CA) of the axoneme of most motile cilia, where they regulate ciliary motility. The external surfaces of both CA microtubules are patterned asymmetrically with large protein complexes that repeat every 16 or 32 nm. The composition of these projections and the mechanisms that establish asymmetry and longitudinal periodicity are unknown. Here, by determining cryo-EM structures of the CA microtubules, we identify 48 different CA-associated proteins, which in turn reveal mechanisms for asymmetric and periodic protein binding to microtubules. We identify arc-MIPs, a novel class of microtubule inner protein, that bind laterally across protofilaments and remodel tubulin structure and lattice contacts. The binding mechanisms utilized by CA proteins may be generalizable to other microtubule-associated proteins. These structures establish a foundation to elucidate the contributions of individual CA proteins to ciliary motility and ciliopathies.
External linksNat Struct Mol Biol / PubMed:35578023 / PubMed Central
MethodsEM (single particle)
Resolution3.7 - 3.8 Å
Structure data

25361

7som

EMDB-25361: Composite map of ciliary C2 central pair apparatus isolated from Chlamydomonas reinhardtii
PDB-7som: Ciliary C2 central pair apparatus isolated from Chlamydomonas reinhardtii
Method: EM (single particle) / Resolution: 3.7 Å

25381

7sqc

EMDB-25381: Composite map of ciliary C1 central pair apparatus isolated from Chlamydomonas reinhardtii
PDB-7sqc: Ciliary C1 central pair apparatus isolated from Chlamydomonas reinhardtii
Method: EM (single particle) / Resolution: 3.8 Å

Chemicals

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

Source
  • chlamydomonas reinhardtii (plant)
KeywordsSTRUCTURAL PROTEIN / cilia / microtubule

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