EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular analysis and essentiality of Aro1 shikimate biosynthesis multi-enzyme in .
ジャーナル・号・ページ	Life Sci Alliance, Vol. 5, Issue 8, Year 2022
掲載日	2022年5月5日
著者	Peter J Stogios / Sean D Liston / Cameron Semper / Bradley Quade / Karolina Michalska / Elena Evdokimova / Shane Ram / Zbyszek Otwinowski / Dominika Borek / Leah E Cowen / Alexei Savchenko /
PubMed 要旨	In the human fungal pathogen , encodes an essential multi-enzyme that catalyses consecutive steps in the shikimate pathway for biosynthesis of chorismate, a precursor to folate and the aromatic ...In the human fungal pathogen , encodes an essential multi-enzyme that catalyses consecutive steps in the shikimate pathway for biosynthesis of chorismate, a precursor to folate and the aromatic amino acids. We obtained the first molecular image of Aro1 that reveals the architecture of all five enzymatic domains and their arrangement in the context of the full-length protein. Aro1 forms a flexible dimer allowing relative autonomy of enzymatic function of the individual domains. Our activity and in cellulo data suggest that only four of Aro1's enzymatic domains are functional and essential for viability of , whereas the 3-dehydroquinate dehydratase (DHQase) domain is inactive because of active site substitutions. We further demonstrate that in , the type II DHQase Dqd1 can compensate for the inactive DHQase domain of Aro1, suggesting an unrecognized essential role for this enzyme in shikimate biosynthesis. In contrast, in and , which do not encode a Dqd1 homolog, Aro1 DHQase domains are enzymatically active, highlighting diversity across species.
リンク	Life Sci Alliance / PubMed:35512834 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.85 - 4.16 Å
構造データ	26357 7u5s EMDB-26357, PDB-7u5s: CryoEM structure of the Candida albicans Aro1 dimer 手法: EM (単粒子) / 解像度: 4.16 Å 26358 7u5t EMDB-26358, PDB-7u5t: Structure of DHQS/EPSPS dimer from Candida albicans Aro1 手法: EM (単粒子) / 解像度: 3.43 Å 26359 7u5u EMDB-26359, PDB-7u5u: Structure of the SK/DHQase/DHSD dimer from Candida albicans Aro1 手法: EM (単粒子) / 解像度: 3.16 Å PDB-6c5c: Crystal structure of the 3-dehydroquinate synthase (DHQS) domain of Aro1 from Candida albicans SC5314 in complex with NADH 手法: X-RAY DIFFRACTION / 解像度: 1.85 Å PDB-7tbu: Crystal structure of the 5-enolpyruvate-shikimate-3-phosphate synthase (EPSPS) domain of Aro1 from Candida albicans in complex with shikimate-3-phosphate 手法: X-RAY DIFFRACTION / 解像度: 1.85 Å PDB-7tbv: Crystal structure of the shikimate kinase + 3-dehydroquinate dehydratase + 3-dehydroshikimate dehydrogenase domains of Aro1 from Candida albicans 手法: X-RAY DIFFRACTION / 解像度: 2.3 Å
化合物	ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NADYM / ニコチンアミドアデニンジヌクレオチド ChemComp-EDO: 1,2-ETHANEDIOL / エチレングリコ-ル / エチレングリコール ChemComp-CL: Unknown entry / クロリド / 塩化物 ChemComp-HOH: WATER / 水 ChemComp-TRS: 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / トリス(ヒドロキシメチル)メチルアンモニウム / pH緩衝剤YM / トリスヒドロキシメチルアミノメタン ChemComp-S3P: SHIKIMATE-3-PHOSPHATE / シキミ酸3-りん酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GOL: GLYCEROL / グリセロ-ル / グリセリン
由来	candida albicans (酵母) candida albicans (strain sc5314 / atcc mya-2876) (酵母) candida albicans ca6 (酵母)
キーワード	LYASE (リアーゼ) / chorismate biosynthesis / Structural Genomics (構造ゲノミクス) / Center for Structural Genomics of Infectious Diseases / CSGID / TRANSFERASE (転移酵素) / OXIDOREDUCTASE (酸化還元酵素) / BIOSYNTHETIC PROTEIN (生合成)