EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Assembly mechanism of the pleomorphic immature poxvirus scaffold.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 1704, Year 2022
掲載日	2022年3月31日
著者	Jaekyung Hyun / Hideyuki Matsunami / Tae Gyun Kim / Matthias Wolf /
PubMed 要旨	In Vaccinia virus (VACV), the prototype poxvirus, scaffold protein D13 forms a honeycomb-like lattice on the viral membrane that results in formation of the pleomorphic immature virion (IV). The ...In Vaccinia virus (VACV), the prototype poxvirus, scaffold protein D13 forms a honeycomb-like lattice on the viral membrane that results in formation of the pleomorphic immature virion (IV). The structure of D13 is similar to those of major capsid proteins that readily form icosahedral capsids in nucleocytoplasmic large DNA viruses (NCLDVs). However, the detailed assembly mechanism of the nonicosahedral poxvirus scaffold has never been understood. Here we show the cryo-EM structures of the D13 trimer and scaffold intermediates produced in vitro. The structures reveal that the displacement of the short N-terminal α-helix is critical for initiation of D13 self-assembly. The continuous curvature of the IV is mediated by electrostatic interactions that induce torsion between trimers. The assembly mechanism explains the semiordered capsid-like arrangement of D13 that is distinct from icosahedral NCLDVs. Our structures explain how a single protein can self-assemble into different capsid morphologies and represent a local exception to the universal Caspar-Klug theory of quasi-equivalence.
リンク	Nat Commun / PubMed:35361762 / PubMed Central
手法	EM (単粒子) / EM (らせん対称)
解像度	2.25 - 7.33 Å
構造データ	31949 7vfd EMDB-31949, PDB-7vfd: Cryo-EM structure of Vaccinia virus scaffolding protein D13 手法: EM (単粒子) / 解像度: 2.25 Å 31950 7vfe EMDB-31950, PDB-7vfe: Cryo-EM structure of Vaccinia virus scaffolding protein D13 with N-terminal polyhistidine tag 手法: EM (単粒子) / 解像度: 2.63 Å 31951 7vff EMDB-31951, PDB-7vff: Cryo-EM structure of Vaccinia virus scaffolding protein D13 with N-terminal 17 residue truncation 手法: EM (単粒子) / 解像度: 4.1 Å 31952 7vfg EMDB-31952, PDB-7vfg: Cryo-EM structure of Vaccinia virus scaffolding protein D13 trimer doublet 手法: EM (単粒子) / 解像度: 3.87 Å EMDB-31953: Cryo-EM structure of Vaccinia virus scaffolding protein D13 tubular assembly 手法: EM (らせん対称) / 解像度: 7.33 Å 31954 7vfh EMDB-31954, PDB-7vfh: Cryo-EM structure of Vaccinia virus scaffolding protein D13 trimer sextet 手法: EM (単粒子) / 解像度: 3.9 Å
化合物	ChemComp-HOH: WATER
由来	Vaccinia virus WR (ウイルス) vaccinia virus (strain western reserve) (ウイルス)
キーワード	VIRAL PROTEIN / Scaffold / capsid / double-jelly-roll