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-Structure paper
Title | Structure of a thylakoid-anchored contractile injection system in multicellular cyanobacteria. |
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Journal, issue, pages | Nat Microbiol, Vol. 7, Issue 3, Page 386-396, Year 2022 |
Publish date | Feb 14, 2022 |
Authors | Gregor L Weiss / Fabian Eisenstein / Ann-Katrin Kieninger / Jingwei Xu / Hannah A Minas / Milena Gerber / Miki Feldmüller / Iris Maldener / Karl Forchhammer / Martin Pilhofer / |
PubMed Abstract | Contractile injection systems (CISs) mediate cell-cell interactions by phage tail-like structures, using two distinct modes of action: extracellular CISs are released into the medium, while type 6 ...Contractile injection systems (CISs) mediate cell-cell interactions by phage tail-like structures, using two distinct modes of action: extracellular CISs are released into the medium, while type 6 secretion systems (T6SSs) are attached to the cytoplasmic membrane and function upon cell-cell contact. Here, we characterized a CIS in the multicellular cyanobacterium Anabaena, with features distinct from extracellular CISs and T6SSs. Cryo-electron tomography of focused ion beam-milled cells revealed that CISs were anchored in thylakoid membrane stacks, facing the cell periphery. Single particle cryo-electron microscopy showed that this unique in situ localization was mediated by extensions of tail fibre and baseplate components. On stress, cyanobacteria induced the formation of ghost cells, presenting thylakoid-anchored CISs to the environment. Functional assays suggest that these CISs may mediate ghost cell formation and/or interactions of ghost cells with other organisms. Collectively, these data provide a framework for understanding the evolutionary re-engineering of CISs and potential roles of these CISs in cyanobacterial programmed cell death. |
External links | Nat Microbiol / PubMed:35165386 / PubMed Central |
Methods | EM (single particle) / EM (tomography) / EM (subtomogram averaging) |
Resolution | 2.8 - 50.0 Å |
Structure data | EMDB-12029, PDB-7b5h: EMDB-12030: EMDB-12031: EMDB-12032: EMDB-12033: EMDB-12034, PDB-7b5i: EMDB-13770: EMDB-13771: EMDB-13772: EMDB-13773: EMDB-13774: EMDB-13775: |
Source |
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Keywords | PROTEIN TRANSPORT / contractile tail / injection system / macromolecular machine / contractile protein |