Jingwei Xu / Charles F Ericson / Yun-Wei Lien / Florentine U N Rutaganira / Fabian Eisenstein / Miki Feldmüller / Nicole King / Martin Pilhofer /
PubMed 要旨
Contractile injection systems (CISs) are phage tail-like nanomachines, mediating bacterial cell-cell interactions as either type VI secretion systems (T6SSs) or extracellular CISs (eCISs). ...Contractile injection systems (CISs) are phage tail-like nanomachines, mediating bacterial cell-cell interactions as either type VI secretion systems (T6SSs) or extracellular CISs (eCISs). Bioinformatic studies uncovered a phylogenetic group of hundreds of putative CIS gene clusters that are highly diverse and widespread; however, only four systems have been characterized. Here we studied a putative CIS gene cluster in the marine bacterium Algoriphagus machipongonensis. Using an integrative approach, we show that the system is compatible with an eCIS mode of action. Our cryo-electron microscopy structure revealed several features that differ from those seen in other CISs: a 'cap adaptor' located at the distal end, a 'plug' exposed to the tube lumen, and a 'cage' formed by massive extensions of the baseplate. These elements are conserved in other CISs, and our genetic tools identified that they are required for assembly, cargo loading and function. Furthermore, our atomic model highlights specific evolutionary hotspots and will serve as a framework for understanding and re-engineering CISs.
EMDB-11734, PDB-7adz: Cryo-EM structure of an extracellular contractile injection system in marine bacterium Algoriphagus machipongonensis, the cap portion in extended state. 手法: EM (単粒子) / 解像度: 2.5 Å
EMDB-11735, PDB-7ae0: Cryo-EM structure of an extracellular contractile injection system in marine bacterium Algoriphagus machipongonensis, the sheath-tube module in extended state. 手法: EM (らせん対称) / 解像度: 2.4 Å
EMDB-11743, PDB-7aeb: Cryo-EM structure of an extracellular contractile injection system in marine bacterium Algoriphagus machipongonensis, the baseplate complex in extended state applied 6-fold symmetry. 手法: EM (単粒子) / 解像度: 2.7 Å
EMDB-11744: Focused refined cryo-EM structure of an extracellular contractile injection system in marine bacterium Algoriphagus machipongonensis, the baseplate complex in extended state applied 6-fold symmetry 手法: EM (単粒子) / 解像度: 2.9 Å
EMDB-11745, PDB-7aef: Cryo-EM structure of an extracellular contractile injection system in marine bacterium Algoriphagus machipongonensis, the baseplate complex in extended state applied 3-fold symmetry. 手法: EM (単粒子) / 解像度: 2.8 Å
EMDB-11746: Cryo-EM structure of the overall extracellular contractile injection system in marine bacterium Algoriphagus machipongonensis 手法: EM (単粒子) / 解像度: 4.4 Å
EMDB-11747, PDB-7aek: Cryo-EM structure of an extracellular contractile injection system in marine bacterium Algoriphagus machipongonensis, the contracted sheath shell. 手法: EM (らせん対称) / 解像度: 2.5 Å
EMDB-11748: Cryo-EM structure of an extracellular contractile injection system in marine bacterium Algoriphagus machipongonensis, the baseplate complex in contracted state. 手法: EM (単粒子) / 解像度: 8.9 Å
EMDB-11749: sub-tomogram averaging of an extracellular contractile injection system in marine bacterium Algoriphagus machipongonensis 手法: EM (サブトモグラム平均) / 解像度: 44.0 Å
EMDB-11750: sub-tomogram averaging of an extracellular contractile injection system mutant (effector deficient mutant) in marine bacterium Algoriphagus machipongonensis 手法: EM (サブトモグラム平均) / 解像度: 32.0 Å
EMDB-13704: cryo electron tomogram of purified AlgoCIS delta Alg16B 手法: EM (トモグラフィー)
EMDB-13705: cryo electron tomogram of Algoriphagus machipongonensis in bacterial late stage 手法: EM (トモグラフィー)
EMDB-13719: Cryo electron tomogram of purified AlgoCIS Cgo1/2 deficient mutant 手法: EM (トモグラフィー)
EMDB-13720: Cryo electron tomogram of purified AlgoCIS Cgo1 deficient mutant 手法: EM (トモグラフィー)
EMDB-13721: Cryo electron tomogram of purified AlgoCIS Cgo2 deficient mutant 手法: EM (トモグラフィー)
EMDB-13722: Cryo electron tomogram of purified AlgoCIS wild-type. 手法: EM (トモグラフィー)
EMDB-13723: Cryo electron sub-tomogram averaging of purified AlgoCIS delta-Alg16B mutant 手法: EM (サブトモグラム平均) / 解像度: 33.0 Å
EMDB-13724: Cryo electron sub-tomogram average of MAC arrays baseplate 手法: EM (サブトモグラム平均) / 解像度: 11.0 Å
EMDB-13725: Cryo electron sub-tomogram average of baseplate in Amoebophilus asiaticus 手法: EM (サブトモグラム平均) / 解像度: 29.0 Å
由来
algoriphagus machipongonensis (バクテリア)
Pseudoalteromonas luteoviolacea (バクテリア)
Candidatus Amoebophilus asiaticus (バクテリア)
キーワード
STRUCTURAL PROTEIN / extracellular contractile injection system
ムービー
コントローラー
構造ビューア
万見文献について
著者
リンク
キーワード
algoriphagus machipongonensis (バクテリア)