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Structure paper

TitleStructural Rearrangements of a Dodecameric Ketol-Acid Reductoisomerase Isolated from a Marine Thermophilic Methanogen.
Journal, issue, pagesBiomolecules, Vol. 11, Year 2021
Publish dateOct 14, 2021 (structure data deposition date)
AuthorsLemaire, O.N. / Muller, M.C. / Kahnt, J. / Wagner, T.
External linksBiomolecules / PubMed:34827677
MethodsX-ray diffraction
Resolution2.1 - 2.2 Å
Structure data

PDB-7q03:
Ketol-acid reductoisomerase from Methanothermococcus thermolithotrophicus in the close state with NADP and Mg2+
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-7q07:
Ketol-acid reductoisomerase from Methanothermococcus thermolithotrophicus in the open state with NADP and tartrate
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-TOE:
2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXYL

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-MG:
Unknown entry

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-TLA:
L(+)-TARTARIC ACID

ChemComp-CL:
Unknown entry

Source
  • methanothermococcus thermolithotrophicus dsm 2095 (archaea)
KeywordsISOMERASE / Ketol-acid reductoisomerase / KARI / methanogenic archaea / conformational rearrangement / native purification / oligomerization / thermophile / branched-chain keto acid / biosynthesis / branched-chain amino acid

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