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Structure paper

TitleDistinct conformations of the HIV-1 V3 loop crown are targetable for broad neutralization.
Journal, issue, pagesNat Commun, Vol. 12, Page 6705-6705, Year 2021
Publish dateDec 2, 2020 (structure data deposition date)
AuthorsFriedrich, N. / Stiegeler, E. / Glogl, M. / Lemmin, T. / Hansen, S. / Kadelka, C. / Wu, Y. / Ernst, P. / Maliqi, L. / Foulkes, C. ...Friedrich, N. / Stiegeler, E. / Glogl, M. / Lemmin, T. / Hansen, S. / Kadelka, C. / Wu, Y. / Ernst, P. / Maliqi, L. / Foulkes, C. / Morin, M. / Eroglu, M. / Liechti, T. / Ivan, B. / Reinberg, T. / Schaefer, J.V. / Karakus, U. / Ursprung, S. / Mann, A. / Rusert, P. / Kouyos, R.D. / Robinson, J.A. / Gunthard, H.F. / Pluckthun, A. / Trkola, A.
External linksNat Commun / PubMed:34795280
MethodsX-ray diffraction
Resolution1.4 - 1.95 Å
Structure data

PDB-7b4t:
Broadly neutralizing DARPin bnD.1 in complex with the HIV-1 envelope variable loop 3 crown mimetic peptide V3-IF (BG505)
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-7b4u:
Broadly neutralizing DARPin bnD.2 in complex with the HIV-1 envelope variable loop 3 crown mimetic peptide V3-IF (BG505)
Method: X-RAY DIFFRACTION / Resolution: 1.45 Å

PDB-7b4v:
Broadly neutralizing DARPin bnD.2 in complex with the HIV-1 envelope variable loop 3 crown mimetic peptide V3-IF (BG505)
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-7b4w:
Broadly neutralizing DARPin bnD.3 in complex with the HIV-1 envelope variable loop 3 crown mimetic peptide V3-IF (BG505)
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-7dne:
DARPin 5m3_D12 in complex with V3-IY (MN) crown mimetic
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-7dnf:
DARPin 63_B7 in complex with V3-IY (MN) crown mimetic
Method: X-RAY DIFFRACTION / Resolution: 1.78 Å

PDB-7dng:
DARPin 63_B7 in complex with linear V3-crown (MN) peptide
Method: X-RAY DIFFRACTION / Resolution: 1.42 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-CA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-NA:
Unknown entry

ChemComp-ACT:
ACETATE ION

Source
  • synthetic construct (others)
  • human immunodeficiency virus 1
KeywordsDE NOVO PROTEIN / HIV / V3 / conformation / neutralization / DARPins / antibodies / designed ankyrin repeat proteins / protein design / protein engineering / anti-HIV

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