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-Structure paper
タイトル | Deterministic chaos in the self-assembly of β sheet nanotubes from an amphipathic oligopeptide. |
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ジャーナル・号・ページ | Matter, Vol. 4, Issue 10, Page 3217-3231, Year 2021 |
掲載日 | 2021年10月6日 |
著者 | Fengbin Wang / Ordy Gnewou / Shengyuan Wang / Tomasz Osinski / Xiaobing Zuo / Edward H Egelman / Vincent P Conticello / |
PubMed 要旨 | The self-assembly of designed peptides into filaments and other higher-order structures has been the focus of intense interest because of the potential for creating new biomaterials and biomedical ...The self-assembly of designed peptides into filaments and other higher-order structures has been the focus of intense interest because of the potential for creating new biomaterials and biomedical devices. These peptide assemblies have also been used as models for understanding biological processes, such as the pathological formation of amyloid. We investigate the assembly of an octapeptide sequence, Ac-FKFEFKFE-NH, motivated by prior studies that demonstrated that this amphipathic β strand peptide self-assembled into fibrils and biocompatible hydrogels. Using high-resolution cryoelectron microscopy (cryo-EM), we are able to determine the atomic structure for two different coexisting forms of the fibrils, containing four and five β sandwich protofilaments, respectively. Surprisingly, the inner walls in both forms are parallel β sheets, while the outer walls are antiparallel β sheets. Our results demonstrate the chaotic nature of peptide self-assembly and illustrate the importance of cryo-EM structural analysis to understand the complex phase behavior of these materials at near-atomic resolution. |
リンク | Matter / PubMed:34632372 / PubMed Central |
手法 | EM (らせん対称) |
解像度 | 3.4 - 3.8 Å |
構造データ | EMDB-23483, PDB-7lqe: EMDB-23484, PDB-7lqf: EMDB-23485, PDB-7lqg: EMDB-23486, PDB-7lqh: EMDB-23487, PDB-7lqi: |
由来 |
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キーワード | PROTEIN FIBRIL / filament / self-assembly peptide filament / Cryo-EM / peptide fibril / nanotube |