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TitleProcessive dynamics of the usher assembly platform during uropathogenic Escherichia coli P pilus biogenesis.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 5207, Year 2021
Publish dateSep 1, 2021
AuthorsMinge Du / Zuanning Yuan / Glenn T Werneburg / Nadine S Henderson / Hemil Chauhan / Amanda Kovach / Gongpu Zhao / Jessica Johl / Huilin Li / David G Thanassi /
PubMed AbstractUropathogenic Escherichia coli assemble surface structures termed pili or fimbriae to initiate infection of the urinary tract. P pili facilitate bacterial colonization of the kidney and ...Uropathogenic Escherichia coli assemble surface structures termed pili or fimbriae to initiate infection of the urinary tract. P pili facilitate bacterial colonization of the kidney and pyelonephritis. P pili are assembled through the conserved chaperone-usher pathway. Much of the structural and functional understanding of the chaperone-usher pathway has been gained through investigations of type 1 pili, which promote binding to the bladder and cystitis. In contrast, the structural basis for P pilus biogenesis at the usher has remained elusive. This is in part due to the flexible and variable-length P pilus tip fiber, creating structural heterogeneity, and difficulties isolating stable P pilus assembly intermediates. Here, we circumvent these hindrances and determine cryo-electron microscopy structures of the activated PapC usher in the process of secreting two- and three-subunit P pilus assembly intermediates, revealing processive steps in P pilus biogenesis and capturing new conformational dynamics of the usher assembly machine.
External linksNat Commun / PubMed:34471127 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 7.6 Å
Structure data

23339

7lhg

EMDB-23339: Cryo-EM map of E. coli P pilus tip assembly intermediate PapC-PapD-PapK-PapG in the first conformation
PDB-7lhg: Cryo-EM structure of E. coli P pilus tip assembly intermediate PapC-PapD-PapK-PapG in the first conformation
Method: EM (single particle) / Resolution: 3.8 Å

23340

7lhh

EMDB-23340: Cryo-EM map of E. coli P pilus tip assembly intermediate PapC-PapD-PapK-PapG in the second conformation
PDB-7lhh: Cryo-EM structure of E. coli P pilus tip assembly intermediate PapC-PapD-PapK-PapG in the second conformation
Method: EM (single particle) / Resolution: 7.2 Å

23341

7lhi

EMDB-23341: Cryo-EM map of E. coli P pilus tip assembly intermediate PapC-PapD-PapK-PapF-PapG
PDB-7lhi: Cryo-EM structure of E. coli P pilus tip assembly intermediate PapC-PapD-PapK-PapF-PapG
Method: EM (single particle) / Resolution: 7.6 Å

Source
  • escherichia coli (E. coli)
KeywordsCHAPERONE / Cryo-EM / Uropathogenic Escherichia coli / chaperone-usher / P pilus

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