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Structure paper

TitleStructure-Activity Relationships in Nonenzymatic Template-Directed RNA Synthesis.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 60, Page 22925-22932, Year 2021
Publish dateNov 25, 2020 (structure data deposition date)
AuthorsGiurgiu, C. / Fang, Z. / Aitken, H.R.M. / Kim, S.C. / Pazienza, L. / Mittal, S. / Szostak, J.W.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:34428345
MethodsX-ray diffraction
Resolution1.44 - 2.281 Å
Structure data

PDB-7kuk:
High resolution RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G
Method: X-RAY DIFFRACTION / Resolution: 1.67 Å

PDB-7kul:
DNA modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G
Method: X-RAY DIFFRACTION / Resolution: 1.64 Å

PDB-7kum:
LNA modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G
Method: X-RAY DIFFRACTION / Resolution: 1.714 Å

PDB-7kun:
2'-F modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G
Method: X-RAY DIFFRACTION / Resolution: 1.58 Å

PDB-7kuo:
FANA modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G
Method: X-RAY DIFFRACTION / Resolution: 2.281 Å

PDB-7kup:
2'-OMe modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G
Method: X-RAY DIFFRACTION / Resolution: 1.44 Å

PDB-7lne:
ANA modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G
Method: X-RAY DIFFRACTION / Resolution: 1.53 Å

PDB-7lnf:
3'-deoxy modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G
Method: X-RAY DIFFRACTION / Resolution: 1.652 Å

PDB-7lng:
TNA modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-GP3:
DIGUANOSINE-5'-TRIPHOSPHATE

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER / Water

ChemComp-LCC:
[(1R,3R,4R,7S)-7-HYDROXY-3-(5-METHYLCYTOSIN-1-YL)-2,5-DIOXABICYCLO[2.2.1]HEPT-1-YL]METHYL DIHYDROGEN PHOSPHATE

ChemComp-LCG:
[(1R,3R,4R,7S)-7-HYDROXY-3-(GUANIN-9-YL)-2,5-DIOXABICYCLO[2.2.1]HEPT-1-YL]METHYL DIHYDROGEN PHOSPHATE

ChemComp-GAO:
GUANINE ARABINOSE-5'-PHOSPHATE

ChemComp-LKC:
4-AMINO-1-[(1S,3R,4R,7S)-7-HYDROXY-1-(HYDROXYMETHYL)-2,5-DIOXABICYCLO[2.2.1]HEPT-3-YL]-5-METHYLPYRIMIDIN-2(1H)-ONE

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-GDO:
3'-deoxy-guanosine 5'-monophosphate

ChemComp-CO:
Unknown entry

ChemComp-TG:
2-azanyl-9-[(2~{R},3~{R},4~{S})-3-oxidanyl-4-[oxidanyl-bis(oxidanylidene)-$l^{6}-phosphanyl]oxy-oxolan-2-yl]-1~{H}-purin-6-one

Source
  • synthetic construct (others)
KeywordsRNA / nonenzymatic RNA extension / DNA

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