Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure of Tau filaments in Prion protein amyloidoses.
Journal, issue, pages	Acta Neuropathol, Vol. 142, Issue 2, Page 227-241, Year 2021
Publish date	Jun 14, 2021
Authors	Grace I Hallinan / Md Rejaul Hoq / Manali Ghosh / Frank S Vago / Anllely Fernandez / Holly J Garringer / Ruben Vidal / Wen Jiang / Bernardino Ghetti /
PubMed Abstract	In human neurodegenerative diseases associated with the intracellular aggregation of Tau protein, the ordered cores of Tau filaments adopt distinct folds. Here, we analyze Tau filaments isolated from ...In human neurodegenerative diseases associated with the intracellular aggregation of Tau protein, the ordered cores of Tau filaments adopt distinct folds. Here, we analyze Tau filaments isolated from the brain of individuals affected by Prion-Protein cerebral amyloid angiopathy (PrP-CAA) with a nonsense mutation in the PRNP gene that leads to early termination of translation of PrP (Q160Ter or Q160X), and Gerstmann-Sträussler-Scheinker (GSS) disease, with a missense mutation in the PRNP gene that leads to an amino acid substitution at residue 198 (F198S) of PrP. The clinical and neuropathologic phenotypes associated with these two mutations in PRNP are different; however, the neuropathologic analyses of these two genetic variants have consistently shown the presence of numerous neurofibrillary tangles (NFTs) made of filamentous Tau aggregates in neurons. We report that Tau filaments in PrP-CAA (Q160X) and GSS (F198S) are composed of 3-repeat and 4-repeat Tau isoforms, having a striking similarity to NFTs in Alzheimer disease (AD). In PrP-CAA (Q160X), Tau filaments are made of both paired helical filaments (PHFs) and straight filaments (SFs), while in GSS (F198S), only PHFs were found. Mass spectrometry analyses of Tau filaments extracted from PrP-CAA (Q160X) and GSS (F198S) brains show the presence of post-translational modifications that are comparable to those seen in Tau aggregates from AD. Cryo-EM analysis reveals that the atomic models of the Tau filaments obtained from PrP-CAA (Q160X) and GSS (F198S) are identical to those of the Tau filaments from AD, and are therefore distinct from those of Pick disease, chronic traumatic encephalopathy, and corticobasal degeneration. Our data support the hypothesis that in the presence of extracellular amyloid deposits and regardless of the primary amino acid sequence of the amyloid protein, similar molecular mechanisms are at play in the formation of identical Tau filaments.
External links	Acta Neuropathol / PubMed:34128081 / PubMed Central
Methods	EM (helical sym.)
Resolution	3.0 - 3.3 Å
Structure data	23871 7mkf EMDB-23871: Paired helical tau filament extracted from PrP-CAA Patient brain tissue \| tau filament \| PHF Tau PDB-7mkf: Paired helical filament extracted from PrP-CAA Patient brain tissue Method: EM (helical sym.) / Resolution: 3.0 Å 23890 7mkg EMDB-23890, PDB-7mkg: Straight tau filament extracted from PrP-CAA Patient brain tissue \| tau filament \| SF Tau Method: EM (helical sym.) / Resolution: 3.07 Å 23894 7mkh EMDB-23894, PDB-7mkh: Paired helical tau filament extracted from GSS Patient brain tissue \| tau filament from Gerstmann Straussler Scheinker disease \| PHF Tau Method: EM (helical sym.) / Resolution: 3.3 Å
Source	homo sapiens (human) Human (human)
Keywords	PROTEIN FIBRIL / tau filament / GSS / PrP-CAA / amyloid fibril / Alzheimer disease