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TitleFirst exposure to the pandemic H1N1 virus induced broadly neutralizing antibodies targeting hemagglutinin head epitopes.
Journal, issue, pagesSci Transl Med, Vol. 13, Issue 596, Year 2021
Publish dateJun 2, 2021
AuthorsJenna J Guthmiller / Julianna Han / Lei Li / Alec W Freyn / Sean T H Liu / Olivia Stovicek / Christopher T Stamper / Haley L Dugan / Micah E Tepora / Henry A Utset / Dalia J Bitar / Natalie J Hamel / Siriruk Changrob / Nai-Ying Zheng / Min Huang / Florian Krammer / Raffael Nachbagauer / Peter Palese / Andrew B Ward / Patrick C Wilson /
PubMed AbstractBroadly neutralizing antibodies are critical for protection against both drifted and shifted influenza viruses. Here, we reveal that first exposure to the 2009 pandemic H1N1 influenza virus recalls ...Broadly neutralizing antibodies are critical for protection against both drifted and shifted influenza viruses. Here, we reveal that first exposure to the 2009 pandemic H1N1 influenza virus recalls memory B cells that are specific to the conserved receptor-binding site (RBS) or lateral patch epitopes of the hemagglutinin (HA) head domain. Monoclonal antibodies (mAbs) generated against these epitopes are broadly neutralizing against H1N1 viruses spanning 40 years of viral evolution and provide potent protection in vivo. Lateral patch-targeting antibodies demonstrated near universal binding to H1 viruses, and RBS-binding antibodies commonly cross-reacted with H3N2 viruses and influenza B viruses. Lateral patch-targeting mAbs were restricted to expressing the variable heavy-chain gene VH3-23 with or without the variable kappa-chain gene VK1-33 and often had a Y-x-R motif within the heavy-chain complementarity determining region 3 to make key contacts with HA. Moreover, lateral patch antibodies that used both VH3-23 and VK1-33 maintained neutralizing capability with recent pH1N1 strains that acquired mutations near the lateral patch. RBS-binding mAbs used a diverse repertoire but targeted the RBS epitope similarly and made extensive contacts with the major antigenic site Sb. Together, our data indicate that RBS- and lateral patch-targeting clones are abundant within the human memory B cell pool, and universal vaccine strategies should aim to drive antibodies against both conserved head and stalk epitopes.
External linksSci Transl Med / PubMed:34078743 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 25.0 Å
Structure data

23792

7mem

EMDB-23792, PDB-7mem:
CryoEM structure of monoclonal Fab 045-09 2B05 binding the lateral patch of influenza virus H1 HA
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-23793:
Negative stain map of monoclonal Fab SFV009 2G01 binding the RBS of H1 HA
Method: EM (single particle) / Resolution: 25.0 Å

EMDB-23794:
Negative stain map of monoclonal Fab 045-09 2B05 binding the lateral patch of H1 HA
Method: EM (single particle) / Resolution: 25.0 Å

EMDB-23795:
Negative stain map of monoclonal Fab SFV019 2A06 binding the lateral patch of H1 HA
Method: EM (single particle) / Resolution: 25.0 Å

EMDB-23796:
Negative stain map of monoclonal Fab SFV015 2F02 binding the lateral patch of H1 HA
Method: EM (single particle) / Resolution: 25.0 Å

EMDB-23797:
Negative stain map of monoclonal Fab 047-09 4G02 binding the lateral patch of H1 HA
Method: EM (single particle) / Resolution: 25.0 Å

EMDB-23798:
Negative stain map of monoclonal Fab 047-09 4B06 binding the lateral patch of H1 HA
Method: EM (single particle) / Resolution: 25.0 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

Source
  • homo sapiens (human)
  • influenza a virus (strain swl a/california/04/2009 h1n1)
KeywordsViral protein/IMMUNE SYSTEM / Hemagglutinin / monoclonal antibody / influenza virus / IMMUNE SYSTEM / Viral protein-IMMUNE SYSTEM complex

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