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TitleMolecular mechanism underlying transport and allosteric inhibition of bicarbonate transporter SbtA.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 118, Issue 22, Year 2021
Publish dateJun 1, 2021
AuthorsSunzhenhe Fang / Xiaowei Huang / Xue Zhang / Minhua Zhang / Yahui Hao / Hui Guo / Lu-Ning Liu / Fang Yu / Peng Zhang /
PubMed AbstractSbtA is a high-affinity, sodium-dependent bicarbonate transporter found in the cyanobacterial CO-concentrating mechanism (CCM). SbtA forms a complex with SbtB, while SbtB allosterically regulates the ...SbtA is a high-affinity, sodium-dependent bicarbonate transporter found in the cyanobacterial CO-concentrating mechanism (CCM). SbtA forms a complex with SbtB, while SbtB allosterically regulates the transport activity of SbtA by binding with adenyl nucleotides. The underlying mechanism of transport and regulation of SbtA is largely unknown. In this study, we report the three-dimensional structures of the cyanobacterial sp. PCC 6803 SbtA-SbtB complex in both the presence and absence of HCO and/or AMP at 2.7 Å and 3.2 Å resolution. An analysis of the inward-facing state of the SbtA structure reveals the HCO/Na binding site, providing evidence for the functional unit as a trimer. A structural comparison found that SbtA adopts an elevator mechanism for bicarbonate transport. A structure-based analysis revealed that the allosteric inhibition of SbtA by SbtB occurs mainly through the T-loop of SbtB, which binds to both the core domain and the scaffold domain of SbtA and locks it in an inward-facing state. T-loop conformation is stabilized by the AMP molecules binding at the SbtB trimer interfaces and may be adjusted by other adenyl nucleotides. The unique regulatory mechanism of SbtA by SbtB makes it important to study inorganic carbon uptake systems in CCM, which can be used to modify photosynthesis in crops.
External linksProc Natl Acad Sci U S A / PubMed:34031249 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.7 - 3.2 Å
Structure data

31135

7egk

EMDB-31135, PDB-7egk:
Bicarbonate transporter complex SbtA-SbtB bound to AMP
Method: EM (single particle) / Resolution: 2.7 Å

PDB-7egl:
Bicarbonate transporter complex SbtA-SbtB bound to HCO3-
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

Chemicals

ChemComp-NA:
Unknown entry

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM

ChemComp-HOH:
WATER

Source
  • Synechocystis sp. PCC 6803 (bacteria)
  • synechocystis sp. (strain pcc 6803 / kazusa) (bacteria)
KeywordsTRANSPORT PROTEIN / Bicarbonate transporter / CO2 concentrating mechanism / Allosteric inhibition / Photosynthesis / CCM

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