Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Cryo-EM structure of metazoan TRAPPIII, the multi-subunit complex that activates the GTPase Rab1.
Journal, issue, pages	EMBO J, Vol. 40, Issue 12, Page e107608, Year 2021
Publish date	Jun 15, 2021
Authors	Antonio Galindo / Vicente J Planelles-Herrero / Gianluca Degliesposti / Sean Munro /
PubMed Abstract	The TRAPP complexes are nucleotide exchange factors that play essential roles in membrane traffic and autophagy. TRAPPII activates Rab11, and TRAPPIII activates Rab1, with the two complexes sharing a ...The TRAPP complexes are nucleotide exchange factors that play essential roles in membrane traffic and autophagy. TRAPPII activates Rab11, and TRAPPIII activates Rab1, with the two complexes sharing a core of small subunits that affect nucleotide exchange but being distinguished by specific large subunits that are essential for activity in vivo. Crystal structures of core subunits have revealed the mechanism of Rab activation, but how the core and the large subunits assemble to form the complexes is unknown. We report a cryo-EM structure of the entire Drosophila TRAPPIII complex. The TRAPPIII-specific subunits TRAPPC8 and TRAPPC11 hold the catalytic core like a pair of tongs, with TRAPPC12 and TRAPPC13 positioned at the joint between them. TRAPPC2 and TRAPPC2L link the core to the two large arms, with the interfaces containing residues affected by disease-causing mutations. The TRAPPC8 arm is positioned such that it would contact Rab1 that is bound to the core, indicating how the arm could determine the specificity of the complex. A lower resolution structure of TRAPPII shows a similar architecture and suggests that the TRAPP complexes evolved from a single ur-TRAPP.
External links	EMBO J / PubMed:34018214 / PubMed Central
Methods	EM (single particle)
Resolution	3.6 - 15.0 Å
Structure data	12052 7b6d EMDB-12052, PDB-7b6d: Drosophila melanogaster TRAPPCore (C1, C2, C2L, C3a/b, C4, C5, C6 subunits) Method: EM (single particle) / Resolution: 4.27 Å 12053 7b6e EMDB-12053: Drosophila melanogaster TRAPP C8, C12 and C13 region map from the TRAPPIII complex PDB-7b6e: Drosophila melanogaster TRAPP C8 subunits region 355 to 661 Method: EM (single particle) / Resolution: 4.5 Å 12054 7b6h EMDB-12054: TRAPP C11 region of the whole TRAPPIII complex PDB-7b6h: Drosophila melanogaster TRAPP C11 subunits region 1 to 718 Method: EM (single particle) / Resolution: 5.4 Å 12056 7b6r EMDB-12056: Drosophila melanogaster TRAPPIII complex PDB-7b6r: Drosophila melanogaster TRAPPIII partial complex: core plus C8 and C11 attached region Method: EM (single particle) / Resolution: 5.8 Å 12057 7b6x EMDB-12057, PDB-7b6x: TRAPPCore from the MiniTRAPPIII complex Method: EM (single particle) / Resolution: 3.6 Å 12058 7b6y EMDB-12058: C8 region from the MiniTRAPPIII complex PDB-7b6y: C8(355-600) from the MiniTRAPPIII complex Method: EM (single particle) / Resolution: 5.7 Å 12060 7b6z EMDB-12060: TRAPPC11 region from the MiniTRAPPIII complex PDB-7b6z: TRAPPC11 subunit (1-716) Method: EM (single particle) / Resolution: 5.5 Å 12063 7b70 EMDB-12063: Whole MiniTRAPPIII PDB-7b70: TRAPPCore plus C8 (355-596) and C11 (1-718) from MiniTRAPPIII Method: EM (single particle) / Resolution: 4.0 Å EMDB-12066: Drosophila melnaogaster TRAPPII Method: EM (single particle) / Resolution: 15.0 Å
Source	drosophila melanogaster (fruit fly)
Keywords	EXOCYTOSIS / Golgi / GEFS / Rab1 / TRAPP / TRAPPIII complex / TRAPP complexes