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TitleStructural Fluctuations of the Human Proteasome α7 Homo-Tetradecamer Double Ring Imply the Proteasomal α-Ring Assembly Mechanism.
Journal, issue, pagesInt J Mol Sci, Vol. 22, Issue 9, Year 2021
Publish dateApr 26, 2021
AuthorsChihong Song / Tadashi Satoh / Taichiro Sekiguchi / Koichi Kato / Kazuyoshi Murata /
PubMed AbstractThe 20S proteasome, which is composed of layered α and β heptameric rings, is the core complex of the eukaryotic proteasome involved in proteolysis. The α7 subunit is a component of the α ring, ...The 20S proteasome, which is composed of layered α and β heptameric rings, is the core complex of the eukaryotic proteasome involved in proteolysis. The α7 subunit is a component of the α ring, and it self-assembles into a homo-tetradecamer consisting of two layers of α7 heptameric rings. However, the structure of the α7 double ring in solution has not been fully elucidated. We applied cryo-electron microscopy to delineate the structure of the α7 double ring in solution, revealing a structure different from the previously reported crystallographic model. The D7-symmetrical double ring was stacked with a 15° clockwise twist and a separation of 3 Å between the two rings. Two more conformations, dislocated and fully open, were also identified. Our observations suggest that the α7 double-ring structure fluctuates considerably in solution, allowing for the insertion of homologous α subunits, finally converting to the hetero-heptameric α rings in the 20S proteasome.
External linksInt J Mol Sci / PubMed:33926037 / PubMed Central
MethodsEM (single particle)
Resolution5.9 - 11.9 Å
Structure data

EMDB-30990, PDB-7e55:
Cryo-EM structure of alpha 7 homo-tetradecamer
Method: EM (single particle) / Resolution: 5.9 Å

EMDB-30991:
Dislocated type of human proteasome alpha7 homo-tetradecamer
Method: EM (single particle) / Resolution: 8.1 Å

EMDB-30992:
Open type of human proteasome alpha 7 homo-tetradecamer
Method: EM (single particle) / Resolution: 11.9 Å

Source
  • homo sapiens (human)
KeywordsLYASE / Conformational fluctuation / double-ring / proteasome / D7 symmetry / CHAPERONE

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