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Structure paper

TitleStructure of the complete, membrane-assembled COPII coat reveals a complex interaction network.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 2034, Year 2021
Publish dateApr 1, 2021
AuthorsJoshua Hutchings / Viktoriya G Stancheva / Nick R Brown / Alan C M Cheung / Elizabeth A Miller / Giulia Zanetti /
PubMed AbstractCOPII mediates Endoplasmic Reticulum to Golgi trafficking of thousands of cargoes. Five essential proteins assemble into a two-layer architecture, with the inner layer thought to regulate coat ...COPII mediates Endoplasmic Reticulum to Golgi trafficking of thousands of cargoes. Five essential proteins assemble into a two-layer architecture, with the inner layer thought to regulate coat assembly and cargo recruitment, and the outer coat forming cages assumed to scaffold membrane curvature. Here we visualise the complete, membrane-assembled COPII coat by cryo-electron tomography and subtomogram averaging, revealing the full network of interactions within and between coat layers. We demonstrate the physiological importance of these interactions using genetic and biochemical approaches. Mutagenesis reveals that the inner coat alone can provide membrane remodelling function, with organisational input from the outer coat. These functional roles for the inner and outer coats significantly move away from the current paradigm, which posits membrane curvature derives primarily from the outer coat. We suggest these interactions collectively contribute to coat organisation and membrane curvature, providing a structural framework to understand regulatory mechanisms of COPII trafficking and secretion.
External linksNat Commun / PubMed:33795673 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution4.6 - 40.0 Å
Structure data

11193

6zg5

EMDB-11193, PDB-6zg5:
COPII on membranes, outer coat right-handed rod, class1
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

11194

6zg6

EMDB-11194, PDB-6zg6:
COPII on membranes, outer coat vertex
Method: EM (subtomogram averaging) / Resolution: 12.0 Å

EMDB-11197:
COPII assembled on membranes, outer coat right-handed rod, class 2
Method: EM (subtomogram averaging) / Resolution: 15.0 Å

EMDB-11198:
COPII assembled on membranes - outer coat extra rod
Method: EM (subtomogram averaging) / Resolution: 15.0 Å

11199

6zga

EMDB-11199, PDB-6zga:
COPII on membranes, inner coat
Method: EM (subtomogram averaging) / Resolution: 4.6 Å

11264

6zl0

EMDB-11264, PDB-6zl0:
COPII on membranes, outer coat left-handed rod
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-ZN:
Unknown entry

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

Source
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • Saccharomyces cerevisiae S288C (yeast)
  • saccharomyces cerevisiae (brewer's yeast)
  • Saccharomyces cerevisiaeaSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
KeywordsPROTEIN TRANSPORT / SECRETION / TRAFFICKING

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