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Structure paper

TitleFrom thiol-subtilisin to omniligase: Design and structure of a broadly applicable peptide ligase.
Journal, issue, pagesComput Struct Biotechnol J, Vol. 19, Page 1277-1287, Year 2021
Publish dateOct 8, 2020 (structure data deposition date)
AuthorsToplak, A. / Teixeira de Oliveira, E.F. / Schmidt, M. / Rozeboom, H.J. / Wijma, H.J. / Meekels, L.K.M. / de Visser, R. / Janssen, D.B. / Nuijens, T.
External linksComput Struct Biotechnol J / PubMed:33717424
MethodsX-ray diffraction
Resolution1.61 - 2.7 Å
Structure data

PDB-7am3:
Crystal structure of Peptiligase mutant - M222P
Method: X-RAY DIFFRACTION / Resolution: 1.61 Å

PDB-7am4:
Crystal structure of Peptiligase mutant - L217H/M222P
Method: X-RAY DIFFRACTION / Resolution: 1.81 Å

PDB-7am5:
Crystal structure of Peptiligase mutant - L217H/M222P/A225N
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-7am6:
Crystal structure of Peptiligase mutant - L217H/M222P/A225N/F189W
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-7am7:
Crystal structure of Peptiligase mutant - M222P/L217H/A225N/F189W/N218D
Method: X-RAY DIFFRACTION / Resolution: 2.61 Å

PDB-7am8:
Crystal structure of Omniligase mutant W189F
Method: X-RAY DIFFRACTION / Resolution: 2.04 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-NA:
Unknown entry

ChemComp-TAR:
D(-)-TARTARIC ACID

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-HIS:
HISTIDINE

ChemComp-AKR:
ACRYLIC ACID

ChemComp-CL:
Unknown entry

Source
  • bacillus amyloliquefaciens (bacteria)
  • hirudo medicinalis (medicinal leech)
KeywordsLIGASE / subtilisin / peptide ligase

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