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TitleMolecular organization of the E. coli cellulose synthase macrocomplex.
Journal, issue, pagesNat Struct Mol Biol, Vol. 28, Issue 3, Page 310-318, Year 2021
Publish dateMar 11, 2021
AuthorsJustin F Acheson / Ruoya Ho / Nicolette F Goularte / Lynette Cegelski / Jochen Zimmer /
PubMed AbstractCellulose is frequently found in communities of sessile bacteria called biofilms. Escherichia coli and other enterobacteriaceae modify cellulose with phosphoethanolamine (pEtN) to promote host tissue ...Cellulose is frequently found in communities of sessile bacteria called biofilms. Escherichia coli and other enterobacteriaceae modify cellulose with phosphoethanolamine (pEtN) to promote host tissue adhesion. The E. coli pEtN cellulose biosynthesis machinery contains the catalytic BcsA-B complex that synthesizes and secretes cellulose, in addition to five other subunits. The membrane-anchored periplasmic BcsG subunit catalyzes pEtN modification. Here we present the structure of the roughly 1 MDa E. coli Bcs complex, consisting of one BcsA enzyme associated with six copies of BcsB, determined by single-particle cryo-electron microscopy. BcsB homo-oligomerizes primarily through interactions between its carbohydrate-binding domains as well as intermolecular beta-sheet formation. The BcsB hexamer creates a half spiral whose open side accommodates two BcsG subunits, directly adjacent to BcsA's periplasmic channel exit. The cytosolic BcsE and BcsQ subunits associate with BcsA's regulatory PilZ domain. The macrocomplex is a fascinating example of cellulose synthase specification.
External linksNat Struct Mol Biol / PubMed:33712813 / PubMed Central
MethodsEM (single particle)
Resolution3.4 - 4.2 Å
Structure data

23146

7l2z

EMDB-23146, PDB-7l2z:
Bacterial cellulose synthase BcsB hexamer
Method: EM (single particle) / Resolution: 3.4 Å

23267

7lby

EMDB-23267, PDB-7lby:
Bacterial cellulose synthase BcsB with polyalanine BcsA model
Method: EM (single particle) / Resolution: 4.2 Å

Source
  • Escherichia coli K-12 (bacteria)
  • escherichia coli (strain k12) (bacteria)
KeywordsBIOSYNTHETIC PROTEIN / bacterial cellulose / periplasmic / structural subunit / TRANSFERASE / synthase

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