Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Virion structure and genome release mechanism of dicistrovirus Kashmir bee virus.
Journal, issue, pages	J Virol, Vol. 95, Issue 11, Year 2021
Publish date	Mar 3, 2021
Authors	Liya Mukhamedova / Tibor Füzik / Jiří Nováček / Dominik Hrebík / Antonín Přidal / Gerardo A Marti / Diego M A Guérin / Pavel Plevka /
PubMed Abstract	Infections of Kashmir bee virus (KBV) are lethal for honeybees and have been associated with colony collapse disorder. KBV and closely related viruses contribute to the ongoing decline in the number ...Infections of Kashmir bee virus (KBV) are lethal for honeybees and have been associated with colony collapse disorder. KBV and closely related viruses contribute to the ongoing decline in the number of honeybee colonies in North America, Europe, Australia, and other parts of the world. Despite the economic and ecological impact of KBV, its structure and infection process remain unknown. Here we present the structure of the virion of KBV determined to a resolution of 2.8 Å. We show that the exposure of KBV to acidic pH induces a reduction in inter-pentamer contacts within capsids and the reorganization of its RNA genome from a uniform distribution to regions of high and low density. Capsids of KBV crack into pieces at acidic pH, resulting in the formation of open particles lacking pentamers of capsid proteins. The large openings of capsids enable the rapid release of genomes and thus limit the probability of their degradation by RNases. The opening of capsids may be a shared mechanism for the genome release of viruses from the family The western honeybee () is indispensable for maintaining agricultural productivity as well as the abundance and diversity of wild flowering plants. However, bees suffer from environmental pollution, parasites, and pathogens, including viruses. Outbreaks of virus infections cause the deaths of individual honeybees as well as collapses of whole colonies. Kashmir bee virus has been associated with colony collapse disorder in the US, and no cure of the disease is currently available. Here we report the structure of an infectious particle of Kashmir bee virus and show how its protein capsid opens to release the genome. Our structural characterization of the infection process determined that therapeutic compounds stabilizing contacts between pentamers of capsid proteins could prevent the genome release of the virus.
External links	J Virol / PubMed:33658338 / PubMed Central
Methods	EM (single particle)
Resolution	2.8 - 21.0 Å
Structure data	12137 7bc3 EMDB-12137, PDB-7bc3: Native virion of Kashmir bee virus at acidic pH Method: EM (single particle) / Resolution: 2.9 Å 12154 7be9 EMDB-12154, PDB-7be9: Kashmir bee virus empty particle at acidic pH Method: EM (single particle) / Resolution: 4.2 Å EMDB-12155: KBV empty particle missing pentamer at acidic pH (C1) Method: EM (single particle) / Resolution: 21.0 Å EMDB-12167: KBV empty particle missing pentamer at acidic pH C5 Method: EM (single particle) / Resolution: 11.0 Å EMDB-12168: KBV empty particle with bulging pentamer at acidic pH Method: EM (single particle) / Resolution: 21.0 Å 12173 7bg8 EMDB-12173, PDB-7bg8: KBV activated particle at acidic pH Method: EM (single particle) / Resolution: 4.0 Å 12182 7bgk EMDB-12182, PDB-7bgk: Native virion of Kashmir bee virus at neutral pH Method: EM (single particle) / Resolution: 2.8 Å
Source	kashmir bee virus Apis mellifera (honey bee)
Keywords	VIRUS / Viruses / Riboviria / Orthornavirae / Pisuviricota / Pisoniviricetes / Picornavirales / Dicistroviridae / Aparavirus / Kashmir Bee Virus