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TitleRegulation of the Dot1 histone H3K79 methyltransferase by histone H4K16 acetylation.
Journal, issue, pagesScience, Vol. 371, Issue 6527, Year 2021
Publish dateJan 22, 2021
AuthorsMarco Igor Valencia-Sánchez / Pablo De Ioannes / Miao Wang / David M Truong / Rachel Lee / Jean-Paul Armache / Jef D Boeke / Karim-Jean Armache /
PubMed AbstractDot1 (disruptor of telomeric silencing-1), the histone H3 lysine 79 (H3K79) methyltransferase, is conserved throughout evolution, and its deregulation is found in human leukemias. Here, we provide ...Dot1 (disruptor of telomeric silencing-1), the histone H3 lysine 79 (H3K79) methyltransferase, is conserved throughout evolution, and its deregulation is found in human leukemias. Here, we provide evidence that acetylation of histone H4 allosterically stimulates yeast Dot1 in a manner distinct from but coordinating with histone H2B ubiquitination (H2BUb). We further demonstrate that this stimulatory effect is specific to acetylation of lysine 16 (H4K16ac), a modification central to chromatin structure. We provide a mechanism of this histone cross-talk and show that H4K16ac and H2BUb play crucial roles in H3K79 di- and trimethylation in vitro and in vivo. These data reveal mechanisms that control H3K79 methylation and demonstrate how H4K16ac, H3K79me, and H2BUb function together to regulate gene transcription and gene silencing to ensure optimal maintenance and propagation of an epigenetic state.
External linksScience / PubMed:33479126 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 4.2 Å
Structure data

22691

7k6p

EMDB-22691, PDB-7k6p:
Active state Dot1 bound to the unacetylated H4 nucleosome
Method: EM (single particle) / Resolution: 3.2 Å

22692

7k6q

EMDB-22692, PDB-7k6q:
Active state Dot1 bound to the H4K16ac nucleosome
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-22693:
Noncatalytic conformation Dot1 bound to the unacetylated H4 nucleosome
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-22694:
Active state Dot1 bound to the H4K16ac nucleosome, Ubiquitin focused map
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-22695:
Active state Dot1 bound to the unacetylated H4 nucleosome, Ubiquitin focused map
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-SAM:
S-ADENOSYLMETHIONINE

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • xenopus laevis (African clawed frog)
  • homo sapiens (human)
  • Synthetic construct (others)
  • Homo sap (brewer's yeast)
  • Synthetic (others)
  • Xenopus lenduensis (Lendu Plateau clawed frog)
KeywordsStructural Protein/DNA/Transferase / TRANSFERASE / Structural Protein-DNA-Transferase complex

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