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TitleCryo-EM structure of the CENP-A nucleosome in complex with phosphorylated CENP-C.
Journal, issue, pagesEMBO J, Vol. 40, Issue 5, Page e105671, Year 2021
Publish dateMar 1, 2021
AuthorsMariko Ariyoshi / Fumiaki Makino / Reito Watanabe / Reiko Nakagawa / Takayuki Kato / Keiichi Namba / Yasuhiro Arimura / Risa Fujita / Hitoshi Kurumizaka / Ei-Ichi Okumura / Masatoshi Hara / Tatsuo Fukagawa /
PubMed AbstractThe CENP-A nucleosome is a key structure for kinetochore assembly. Once the CENP-A nucleosome is established in the centromere, additional proteins recognize the CENP-A nucleosome to form a ...The CENP-A nucleosome is a key structure for kinetochore assembly. Once the CENP-A nucleosome is established in the centromere, additional proteins recognize the CENP-A nucleosome to form a kinetochore. CENP-C and CENP-N are CENP-A binding proteins. We previously demonstrated that vertebrate CENP-C binding to the CENP-A nucleosome is regulated by CDK1-mediated CENP-C phosphorylation. However, it is still unknown how the phosphorylation of CENP-C regulates its binding to CENP-A. It is also not completely understood how and whether CENP-C and CENP-N act together on the CENP-A nucleosome. Here, using cryo-electron microscopy (cryo-EM) in combination with biochemical approaches, we reveal a stable CENP-A nucleosome-binding mode of CENP-C through unique regions. The chicken CENP-C structure bound to the CENP-A nucleosome is stabilized by an intramolecular link through the phosphorylated CENP-C residue. The stable CENP-A-CENP-C complex excludes CENP-N from the CENP-A nucleosome. These findings provide mechanistic insights into the dynamic kinetochore assembly regulated by CDK1-mediated CENP-C phosphorylation.
External linksEMBO J / PubMed:33463726 / PubMed Central
MethodsEM (single particle)
Resolution4.2 - 7.8 Å
Structure data

30237

7bxt

EMDB-30237, PDB-7bxt:
The cryo-EM structure of CENP-A nucleosome in complex with CENP-C peptide and CENP-N N-terminal domain
Method: EM (single particle) / Resolution: 4.2 Å

30239

7by0

EMDB-30239, PDB-7by0:
The cryo-EM structure of CENP-A nucleosome in complex with the phosphorylated CENP-C
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-30240:
The cryo-EM density map of CENP-A nucleosome in complex with CENP-C C-terminal domain
Method: EM (single particle) / Resolution: 6.8 Å

EMDB-30241:
The cryo-EM density map of CENP-A nucleosome in complex with asymmetric CENP-C C-terminal domain and CENP-N N-terminal domain
Method: EM (single particle) / Resolution: 7.8 Å

Source
  • Ectocarpus siliculosus (eukaryote)
  • homo sapiens (human)
  • Escherichia coli (strain B / BL21-DE3) (bacteria)
  • gallus gallus (chicken)
  • synthetic construct (others)
  • unidentified cloning vector (others)
KeywordsCELL CYCLE/DNA / CENP-A nucleosome / CENP-C / CENP-N / complex / kinetochore / NUCLEAR PROTEIN / CELL CYCLE-DNA complex / CELL CYCLE

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