Robert N Kirchdoerfer / Mahesh Bhandari / Olnita Martini / Leigh M Sewall / Sandhya Bangaru / Kyoung-Jin Yoon / Andrew B Ward /
PubMed Abstract
Porcine epidemic diarrhea virus (PEDV) is an alphacoronavirus responsible for significant morbidity and mortality in pigs. A key determinant of viral tropism and entry, the PEDV spike protein is a ...Porcine epidemic diarrhea virus (PEDV) is an alphacoronavirus responsible for significant morbidity and mortality in pigs. A key determinant of viral tropism and entry, the PEDV spike protein is a key target for the host antibody response and a good candidate for a protein-based vaccine immunogen. We used electron microscopy to evaluate the PEDV spike structure, as well as pig polyclonal antibody responses to viral infection. The structure of the PEDV spike reveals a configuration similar to that of HuCoV-NL63. Several PEDV protein-protein interfaces are mediated by non-protein components, including a glycan at Asn264 and two bound palmitoleic acid molecules. The polyclonal antibody response to PEDV infection shows a dominance of epitopes in the S1 region. This structural and immune characterization provides insights into coronavirus spike stability determinants and explores the immune landscape of viral spike proteins.
EMDB-21391, PDB-6vv5: Cryo-EM structure of porcine epidemic diarrhea virus (PEDV) spike protein Method: EM (single particle) / Resolution: 3.5 Å
EMDB-21392: Porcine epidemic diarrhea virus (PEDV) spike protein, expressed in Sf9 cells and negatively stained Method: EM (single particle) / Resolution: 13.0 Å
EMDB-21393: Porcine epidemic diarrhea virus (PEDV) spike protein, expressed in 293F cells and negatively stained Method: EM (single particle) / Resolution: 12.0 Å
EMDB-21394: Porcine epidemic diarrhea virus (PEDV) spike protein, expressed in 293F cells with kifunensine and negatively stained Method: EM (single particle) / Resolution: 10.0 Å
EMDB-21395: Porcine epidemic diarrhea virus (PEDV) spike protein, expressed in 293S cells and negatively stained Method: EM (single particle) / Resolution: 19.0 Å
EMDB-21396: Porcine epidemic diarrhea virus (PEDV) spike protein with N264D mutation, expressed in 293F cells and negatively stained Method: EM (single particle) / Resolution: 18.0 Å
EMDB-21397: Porcine epidemic diarrhea virus (PEDV) spike protein, N-terminal domain deletion, expressed in Sf9 cells and negatively stained Method: EM (single particle) / Resolution: 23.0 Å
EMDB-21398: Porcine epidemic diarrhea virus (PEDV) spike protein bound to negative control polyclonal Fab Method: EM (single particle) / Resolution: 15.0 Å
EMDB-21399: Porcine epidemic diarrhea virus (PEDV) spike protein bound to polyclonal Fab sample 1, side-binding Fab Method: EM (single particle) / Resolution: 22.0 Å
EMDB-21400: Porcine epidemic diarrhea virus (PEDV) spike protein bound to polyclonal Fab sample 1, apex-binding Fab Method: EM (single particle) / Resolution: 28.0 Å
EMDB-21401: Porcine epidemic diarrhea virus (PEDV) spike protein bound to polyclonal Fab sample 2, side-binding Fab Method: EM (single particle) / Resolution: 23.0 Å
EMDB-21402: Porcine epidemic diarrhea virus (PEDV) spike protein bound to polyclonal Fab sample 2, apex-binding Fab Method: EM (single particle) / Resolution: 23.0 Å
EMDB-21403: Porcine epidemic diarrhea virus (PEDV) spike protein bound to polyclonal Fab sample 3, side-binding Fab Method: EM (single particle) / Resolution: 15.0 Å
EMDB-21404: Porcine epidemic diarrhea virus (PEDV) spike protein bound to polyclonal Fab sample 3, apex-binding Fab Method: EM (single particle) / Resolution: 17.0 Å
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VIRAL PROTEIN / 
