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Structure paper

TitleCrystallographic binding studies of rat peroxisomal multifunctional enzyme type 1 with 3-ketodecanoyl-CoA: capturing active and inactive states of its hydratase and dehydrogenase catalytic sites.
Journal, issue, pagesActa Crystallogr D Struct Biol, Vol. 76, Page 1256-1269, Year 2020
Publish dateAug 1, 2017 (structure data deposition date)
AuthorsSridhar, S. / Schmitz, W. / Hiltunen, J.K. / Venkatesan, R. / Bergmann, U. / Kiema, T.R. / Wierenga, R.K.
External linksActa Crystallogr D Struct Biol / PubMed:33263331
MethodsX-ray diffraction
Resolution1.7 - 2.49 Å
Structure data

PDB-5omo:
CRYSTAL STRUCTURE OF RAT PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE-1 (RPMFE1) COMPLEXED WITH WITH 3S-HYDROXY-DECANOYL-COA AND 3-KETO-DECANOYL-COA
Method: X-RAY DIFFRACTION / Resolution: 2.49 Å

PDB-6z5f:
CRYSTAL STRUCTURE OF RAT PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE-1 (RPMFE1) COMPLEXED WITH 3-KETODECANOYL-COA AND OXIDISED NICOTINAMIDE ADENINE DINUCLEOTIDE
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

PDB-6z5o:
CRYSTAL STRUCTURE OF RAT PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE-1 (RPMFE1) COMPLEXED WITH COENZYME-A AND OXIDISED NICOTINAMIDE ADENINE DINUCLEOTIDE
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-6z5v:
CRYSTAL STRUCTURE OF RAT PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE-1 (RPMFE1) COMPLEXED WITH 3-KETODECANOYL-COA IN CROTONASE FOLD AND OXIDISED NICOTINAMIDE ADENINE DINUCLEOTIDE IN HAD FOLD
Method: X-RAY DIFFRACTION / Resolution: 2.33 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-HSC:
(S)-3-HYDROXYDECANOYL-COA

ChemComp-ZOZ:
3-KETO-DECANOYL-COA

ChemComp-HOH:
WATER

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-COA:
COENZYME A

ChemComp-NCA:
NICOTINAMIDE / medication*YM

Source
  • rattus norvegicus (Norway rat)
KeywordsOXIDOREDUCTASE / 3S-HYDROXY-DECANOYL-COA / 3-KETO-DECANOYL-COA / MFE1 / BETA-OXIDATION / FATTY ACID / CROTONASE / 3-HYDROXYACYL-COA- DEHYDROGENASE / HYDRATASE / 3-KETODECANOYL-COA / peroxisome / COENZYME-A / BETA OXIDATION / 3S-hydroxyacyl-CoA-dehydrogenase / PEROXISOME.

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