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TitleThe cryo-EM structure of the endocytic receptor DEC-205.
Journal, issue, pagesJ Biol Chem, Vol. 296, Page 100127, Year 2021
Publish dateDec 3, 2020
AuthorsBenjamin S Gully / Hariprasad Venugopal / Alex J Fulcher / Zhihui Fu / Jessica Li / Felix A Deuss / Carmen Llerena / William R Heath / Mireille H Lahoud / Irina Caminschi / Jamie Rossjohn / Richard Berry /
PubMed AbstractDEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine ...DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine oligonucleotides, a motif often seen in bacterial or viral DNA. However, despite growing biological and clinical significance, little is known about the structural arrangement of this receptor or any of its family members. Here, we describe the 3.2 Å cryo-EM structure of human DEC-205, thereby illuminating the structure of the mannose receptor protein family. The DEC-205 monomer forms a compact structure comprising two intercalated rings of C-type lectin-like domains, where the N-terminal cysteine-rich and fibronectin domains reside at the central intersection. We establish a pH-dependent oligomerization pathway forming tetrameric DEC-205 using solution-based techniques and ultimately solved the 4.9 Å cryo-EM structure of the DEC-205 tetramer to identify the unfurling of the second lectin ring which enables tetramer formation. Furthermore, we suggest the relevance of this oligomerization pathway within a cellular setting, whereby cytosine-guanosine binding appeared to disrupt this cell-surface oligomer. Accordingly, we provide insight into the structure and oligomeric assembly of the DEC-205 receptor.
External linksJ Biol Chem / PubMed:33257321 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 5.0 Å
Structure data

22422

7jpt

EMDB-22422, PDB-7jpt:
Structure of an endocytic receptor
Method: EM (single particle) / Resolution: 3.2 Å

22423

7jpu

EMDB-22423, PDB-7jpu:
Structure of an endocytic receptor
Method: EM (single particle) / Resolution: 5.0 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

Source
  • homo sapiens (human)
KeywordsIMMUNE SYSTEM / Cell-surface receptor / Immune receptor / mannose receptor family

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