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TitleCryo-EM structures of intact V-ATPase from bovine brain.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 3921, Year 2020
Publish dateAug 6, 2020
AuthorsRong Wang / Tao Long / Abdirahman Hassan / Jin Wang / Yingyuan Sun / Xiao-Song Xie / Xiaochun Li /
PubMed AbstractThe vacuolar-type H-ATPases (V-ATPase) hydrolyze ATP to pump protons across the plasma or intracellular membrane, secreting acids to the lumen or acidifying intracellular compartments. It has been ...The vacuolar-type H-ATPases (V-ATPase) hydrolyze ATP to pump protons across the plasma or intracellular membrane, secreting acids to the lumen or acidifying intracellular compartments. It has been implicated in tumor metastasis, renal tubular acidosis, and osteoporosis. Here, we report two cryo-EM structures of the intact V-ATPase from bovine brain with all the subunits including the subunit H, which is essential for ATPase activity. Two type-I transmembrane proteins, Ac45 and (pro)renin receptor, along with subunit c", constitute the core of the c-ring. Three different conformations of A/B heterodimers suggest a mechanism for ATP hydrolysis that triggers a rotation of subunits DF, inducing spinning of subunit d with respect to the entire c-ring. Moreover, many lipid molecules have been observed in the Vo domain to mediate the interactions between subunit c, c", (pro)renin receptor, and Ac45. These two structures reveal unique features of mammalian V-ATPase and suggest a mechanism of V1-Vo torque transmission.
External linksNat Commun / PubMed:32764564 / PubMed Central
MethodsEM (single particle)
Resolution3.37 - 3.79 Å
Structure data

22121

6xbw

EMDB-22121, PDB-6xbw:
Cryo-EM structure of V-ATPase from bovine brain, state 1
Method: EM (single particle) / Resolution: 3.37 Å

22122

6xby

EMDB-22122, PDB-6xby:
Cryo-EM structure of V-ATPase from bovine brain, state 2
Method: EM (single particle) / Resolution: 3.79 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-POV:
(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / phospholipid*YM

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-OLA:
OLEIC ACID

Source
  • bos taurus (cattle)
KeywordsPROTON TRANSPORT

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