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Structure paper

TitleConstruction of the Central Protuberance and L1 Stalk during 60S Subunit Biogenesis.
Journal, issue, pagesMol Cell, Vol. 79, Issue 4, Page 615-628.e5, Year 2020
Publish dateAug 20, 2020
AuthorsLukas Kater / Valentin Mitterer / Matthias Thoms / Jingdong Cheng / Otto Berninghausen / Roland Beckmann / Ed Hurt /
PubMed AbstractRibosome assembly is driven by numerous assembly factors, including the Rix1 complex and the AAA ATPase Rea1. These two assembly factors catalyze 60S maturation at two distinct states, triggering ...Ribosome assembly is driven by numerous assembly factors, including the Rix1 complex and the AAA ATPase Rea1. These two assembly factors catalyze 60S maturation at two distinct states, triggering poorly understood large-scale structural transitions that we analyzed by cryo-electron microscopy. Two nuclear pre-60S intermediates were discovered that represent previously unknown states after Rea1-mediated removal of the Ytm1-Erb1 complex and reveal how the L1 stalk develops from a pre-mature nucleolar to a mature-like nucleoplasmic state. A later pre-60S intermediate shows how the central protuberance arises, assisted by the nearby Rix1-Rea1 machinery, which was solved in its pre-ribosomal context to molecular resolution. This revealed a Rix1-Ipi3 tetramer anchored to the pre-60S via Ipi1, strategically positioned to monitor this decisive remodeling. These results are consistent with a general underlying principle that temporarily stabilized immature RNA domains are successively remodeled by assembly factors, thereby ensuring failsafe assembly progression.
External linksMol Cell / PubMed:32668200
MethodsEM (single particle)
Resolution3.0 - 4.2 Å
Structure data

10836

6yle

EMDB-10836, PDB-6yle:
Rix1-Rea1 pre-60S particle - Rix1-subcomplex, body 3 (rigid body refinement)
Method: EM (single particle) / Resolution: 3.3 Å

10837

6ylf

EMDB-10837, PDB-6ylf:
Rix1-Rea1 pre-60S particle - Rea1, body 3 (rigid body refinement, composite structure of Rea1 ring and tail)
Method: EM (single particle) / Resolution: 4.2 Å

10838

6ylg

EMDB-10838, PDB-6ylg:
Rix1-Rea1 pre-60S particle - 60S core, body 1 (rigid body refinement)
Method: EM (single particle) / Resolution: 3.0 Å

10839

6ylh

EMDB-10839, PDB-6ylh:
Rix1-Rea1 pre-60S particle - full composite structure
Method: EM (single particle) / Resolution: 3.1 Å

10841

6ylx

EMDB-10841, PDB-6ylx:
pre-60S State NE1 (TAP-Flag-Nop53)
Method: EM (single particle) / Resolution: 3.9 Å

10842

6yly

EMDB-10842, PDB-6yly:
pre-60S State NE2 (TAP-Flag-Nop53)
Method: EM (single particle) / Resolution: 3.8 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • Baker's yeast (brewer's yeast)
KeywordsRIBOSOME / pre-60S / Biogenesis / LSU / Large subunit / ribosome assembly

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