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TitleMZT Proteins Form Multi-Faceted Structural Modules in the γ-Tubulin Ring Complex.
Journal, issue, pagesCell Rep, Vol. 31, Issue 13, Page 107791, Year 2020
Publish dateJun 30, 2020
AuthorsMichal Wieczorek / Tzu-Lun Huang / Linas Urnavicius / Kuo-Chiang Hsia / Tarun M Kapoor /
PubMed AbstractMicrotubule organization depends on the γ-tubulin ring complex (γ-TuRC), a ∼2.3-MDa nucleation factor comprising an asymmetric assembly of γ-tubulin and GCP2-GCP6. However, it is currently ...Microtubule organization depends on the γ-tubulin ring complex (γ-TuRC), a ∼2.3-MDa nucleation factor comprising an asymmetric assembly of γ-tubulin and GCP2-GCP6. However, it is currently unclear how the γ-TuRC-associated microproteins MZT1 and MZT2 contribute to the structure and regulation of the holocomplex. Here, we report cryo-EM structures of MZT1 and MZT2 in the context of the native human γ-TuRC. MZT1 forms two subcomplexes with the N-terminal α-helical domains of GCP3 or GCP6 (GCP-NHDs) within the γ-TuRC "lumenal bridge." We determine the X-ray structure of recombinant MZT1/GCP6-NHD and find it is similar to that within the native γ-TuRC. We identify two additional MZT/GCP-NHD-like subcomplexes, one of which is located on the outer face of the γ-TuRC and comprises MZT2 and GCP2-NHD in complex with a centrosomin motif 1 (CM1)-containing peptide. Our data reveal how MZT1 and MZT2 establish multi-faceted, structurally mimetic "modules" that can expand structural and regulatory interfaces in the γ-TuRC.
External linksCell Rep / PubMed:32610146 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution3.29434890409 - 4.5 Å
Structure data

21984

6x0u

EMDB-21984, PDB-6x0u:
Structure of MZT1/GCP3-NHD and MZT1/GCP6-NHD in the gamma-TuRC lumenal bridge
Method: EM (single particle) / Resolution: 3.6 Å

21985

6x0v

EMDB-21985, PDB-6x0v:
Structure of MZT2/GCP-NHD and CDK5Rap2 at position 13 of the gamma-TuRC
Method: EM (single particle) / Resolution: 4.5 Å

PDB-6m33:
Crystal structure of Homo Sapian GCP6 N-terminus and Mozart1
Method: X-RAY DIFFRACTION / Resolution: 3.29434890409 Å

Source
  • homo sapiens (human)
KeywordsCELL CYCLE / Gamma tubulin complex / microtubule / GCP / Mzt1 / STRUCTURAL PROTEIN / gamma-TuRC / lumenal bridge / MZT2 / CDK5Rap2

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