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Structure paper

TitleCryo-EM structures of inactive and active GABA receptor.
Journal, issue, pagesCell Res, Vol. 30, Issue 7, Page 564-573, Year 2020
Publish dateJun 3, 2020
AuthorsChunyou Mao / Cangsong Shen / Chuntao Li / Dan-Dan Shen / Chanjuan Xu / Shenglan Zhang / Rui Zhou / Qingya Shen / Li-Nan Chen / Zhinong Jiang / Jianfeng Liu / Yan Zhang /
PubMed AbstractMetabotropic GABA G protein-coupled receptor functions as a mandatory heterodimer of GB1 and GB2 subunits and mediates inhibitory neurotransmission in the central nervous system. Each subunit is ...Metabotropic GABA G protein-coupled receptor functions as a mandatory heterodimer of GB1 and GB2 subunits and mediates inhibitory neurotransmission in the central nervous system. Each subunit is composed of the extracellular Venus flytrap (VFT) domain and transmembrane (TM) domain. Here we present cryo-EM structures of full-length human heterodimeric GABA receptor in the antagonist-bound inactive state and in the active state complexed with an agonist and a positive allosteric modulator in the presence of G protein at a resolution range of 2.8-3.0 Å. Our structures reveal that agonist binding stabilizes the closure of GB1 VFT, which in turn triggers a rearrangement of TM interfaces between the two subunits from TM3-TM5/TM3-TM5 in the inactive state to TM6/TM6 in the active state and finally induces the opening of intracellular loop 3 and synergistic shifting of TM3, 4 and 5 helices in GB2 TM domain to accommodate the α5-helix of G. We also observed that the positive allosteric modulator anchors at the dimeric interface of TM domains. These results provide a structural framework for understanding class C GPCR activation and a rational template for allosteric modulator design targeting the dimeric interface of GABA receptor.
External linksCell Res / PubMed:32494023 / PubMed Central
MethodsEM (single particle)
Resolution2.9 - 3.0 Å
Structure data

30300

7c7q

EMDB-30300, PDB-7c7q:
Cryo-EM structure of the baclofen/BHFF-bound human GABA(B) receptor in active state
Method: EM (single particle) / Resolution: 3.0 Å

30301

7c7s

EMDB-30301, PDB-7c7s:
Cryo-EM structure of the CGP54626-bound human GABA(B) receptor in inactive state.
Method: EM (single particle) / Resolution: 2.9 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-FN0:
(3S)-5,7-ditert-butyl-3-oxidanyl-3-(trifluoromethyl)-1-benzofuran-2-one

ChemComp-2C0:
baclofen / medication*YM / Baclofen

ChemComp-2BV:
(R)-(cyclohexylmethyl)[(2S)-3-{[(1S)-1-(3,4-dichlorophenyl)ethyl]amino}-2-hydroxypropyl]phosphinic acid

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / GABAB / Cryo-EM / GPCR / active / PAM

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