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TitlePhage G Structure at 6.1 Å Resolution, Condensed DNA, and Host Identity Revision to a Lysinibacillus.
Journal, issue, pagesJ Mol Biol, Vol. 432, Issue 14, Page 4139-4153, Year 2020
Publish dateJun 26, 2020
AuthorsBrenda González / Lyman Monroe / Kunpeng Li / Rui Yan / Elena Wright / Thomas Walter / Daisuke Kihara / Susan T Weintraub / Julie A Thomas / Philip Serwer / Wen Jiang /
PubMed AbstractPhage G has the largest capsid and genome of any known propagated phage. Many aspects of its structure, assembly, and replication have not been elucidated. Herein, we present the dsDNA-packed and ...Phage G has the largest capsid and genome of any known propagated phage. Many aspects of its structure, assembly, and replication have not been elucidated. Herein, we present the dsDNA-packed and empty phage G capsid at 6.1 and 9 Å resolution, respectively, using cryo-EM for structure determination and mass spectrometry for protein identification. The major capsid protein, gp27, is identified and found to share the HK97-fold universally conserved in all previously solved dsDNA phages. Trimers of the decoration protein, gp26, sit on the 3-fold axes and are thought to enhance the interactions of the hexameric capsomeres of gp27, for other phages encoding decoration proteins. Phage G's decoration protein is longer than what has been reported in other phages, and we suspect the extra interaction surface area helps stabilize the capsid. We identified several additional capsid proteins, including a candidate for the prohead protease responsible for processing gp27. Furthermore, cryo-EM reveals a range of partially full, condensed DNA densities that appear to have no contact with capsid shell. Three analyses confirm that the phage G host is a Lysinibacillus, and not Bacillus megaterium: identity of host proteins in our mass spectrometry analyses, genome sequence of the phage G host, and host range of phage G.
External linksJ Mol Biol / PubMed:32454153 / PubMed Central
MethodsEM (single particle)
Resolution6.1 - 9.0 Å
Structure data

21695

6wkk

EMDB-21695: Full phage G capsid cryoEM structure at 6.1 Angstrom resolution
PDB-6wkk: Phage G gp27 major capsid proteins and gp26 decoration proteins
Method: EM (single particle) / Resolution: 6.1 Å

EMDB-21702:
Empty phage G cryoEM capsid structure at 9 Angstrom resolution
Method: EM (single particle) / Resolution: 9.0 Å

Source
  • bacillus virus g
KeywordsVIRUS / phage G / major capsid protein / decoration protein / capsid / icosahedral / gp26 / gp27

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