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TitleVisualization of two architectures in class-II CAP-dependent transcription activation.
Journal, issue, pagesPLoS Biol, Vol. 18, Issue 4, Page e3000706, Year 2020
Publish dateApr 20, 2020
AuthorsWei Shi / Yanan Jiang / Yibin Deng / Zigang Dong / Bin Liu /
PubMed AbstractTranscription activation by cyclic AMP (cAMP) receptor protein (CAP) is the classic paradigm of transcription regulation in bacteria. CAP was suggested to activate transcription on class-II promoters ...Transcription activation by cyclic AMP (cAMP) receptor protein (CAP) is the classic paradigm of transcription regulation in bacteria. CAP was suggested to activate transcription on class-II promoters via a recruitment and isomerization mechanism. However, whether and how it modifies RNA polymerase (RNAP) to initiate transcription remains unclear. Here, we report cryo-electron microscopy (cryo-EM) structures of an intact Escherichia coli class-II CAP-dependent transcription activation complex (CAP-TAC) with and without de novo RNA transcript. The structures reveal two distinct architectures of TAC and raise the possibility that CAP binding may induce substantial conformational changes in all the subunits of RNAP and transiently widen the main cleft of RNAP to facilitate DNA promoter entering and formation of the initiation open complex. These structural changes vanish during further RNA transcript synthesis. The observations in this study may reveal a possible on-pathway intermediate and suggest a possibility that CAP activates transcription by inducing intermediate state, in addition to the previously proposed stabilization mechanism.
External linksPLoS Biol / PubMed:32310937 / PubMed Central
MethodsEM (single particle)
Resolution4.29 - 4.52 Å
Structure data

20286

6pb4

EMDB-20286, PDB-6pb4:
The E. coli class-II CAP-dependent transcription activation complex with de novo RNA transcript at the state 2
Method: EM (single particle) / Resolution: 4.35 Å

20287

6pb5

EMDB-20287, PDB-6pb5:
The E. coli class-II CAP-dependent transcription activation complex at the state 1 architecture
Method: EM (single particle) / Resolution: 4.52 Å

20288

6pb6

EMDB-20288, PDB-6pb6:
The E. coli class-II CAP-dependent transcription activation complex at the state 2
Method: EM (single particle) / Resolution: 4.29 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-CMP:
ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE

Source
  • escherichia coli (E. coli)
KeywordsTRANSCRIPTION/DNA/RNA / class-II / transcription activation complex / CAP-dependent / de novo RNA synthesis / TRANSCRIPTION / TRANSCRIPTION-DNA-RNA complex / TRANSCRIPTION/DNA / TRANSCRIPTION-DNA complex / state 2 architecture

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