Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	A Structure-Based Model for the Complete Transcription Cycle of Influenza Polymerase.
Journal, issue, pages	Cell, Vol. 181, Issue 4, Page 877-893.e21, Year 2020
Publish date	May 14, 2020
Authors	Joanna M Wandzik / Tomas Kouba / Manikandan Karuppasamy / Alexander Pflug / Petra Drncova / Jan Provaznik / Nayara Azevedo / Stephen Cusack /
PubMed Abstract	Influenza polymerase uses unique mechanisms to synthesize capped and polyadenylated mRNAs from the genomic viral RNA (vRNA) template, which is packaged inside ribonucleoprotein particles (vRNPs). ...Influenza polymerase uses unique mechanisms to synthesize capped and polyadenylated mRNAs from the genomic viral RNA (vRNA) template, which is packaged inside ribonucleoprotein particles (vRNPs). Here, we visualize by cryoelectron microscopy the conformational dynamics of the polymerase during the complete transcription cycle from pre-initiation to termination, focusing on the template trajectory. After exiting the active site cavity, the template 3' extremity rebinds into a specific site on the polymerase surface. Here, it remains sequestered during all subsequent transcription steps, forcing the template to loop out as it further translocates. At termination, the strained connection between the bound template 5' end and the active site results in polyadenylation by stuttering at uridine 17. Upon product dissociation, further conformational changes release the trapped template, allowing recycling back into the pre-initiation state. Influenza polymerase thus performs transcription while tightly binding to and protecting both template ends, allowing efficient production of multiple mRNAs from a single vRNP.
External links	Cell / PubMed:32304664
Methods	EM (single particle) / X-ray diffraction
Resolution	2.41 - 3.52 Å
Structure data	10354 6szu EMDB-10354, PDB-6szu: Bat Influenza A polymerase pre-termination complex with pyrophosphate using 44-mer vRNA template with mutated oligo(U) sequence Method: EM (single particle) / Resolution: 2.41 Å 10355 6szv EMDB-10355, PDB-6szv: Bat Influenza A polymerase elongation complex with incoming UTP analogue (core + endonuclease only) Method: EM (single particle) / Resolution: 2.5 Å 10356 6t0n EMDB-10356, PDB-6t0n: Bat Influenza A polymerase pre-initiation complex Method: EM (single particle) / Resolution: 2.54 Å 10357 6t0r EMDB-10357, PDB-6t0r: Bat Influenza A polymerase product dissociation complex using 44-mer vRNA template with mutated oligo(U) sequence Method: EM (single particle) / Resolution: 2.82 Å 10358 6t0s EMDB-10358, PDB-6t0s: Bat Influenza A polymerase stuttering complex using 44-mer vRNA template with intact oligo(U) sequence Method: EM (single particle) / Resolution: 3.04 Å 10359 6t0u EMDB-10359, PDB-6t0u: Bat Influenza A polymerase product dissociation complex using 44-mer vRNA template with intact oligo(U) sequence Method: EM (single particle) / Resolution: 3.12 Å 10360 6t0v EMDB-10360, PDB-6t0v: Bat Influenza A polymerase elongation complex with incoming UTP analogue (complete polymerase) Method: EM (single particle) / Resolution: 3.02 Å 10361 6t0w EMDB-10361, PDB-6t0w: Human Influenza B polymerase recycling complex Method: EM (single particle) / Resolution: 3.18 Å 10368 6t2c EMDB-10368, PDB-6t2c: Bat Influenza A polymerase recycling complex Method: EM (single particle) / Resolution: 3.52 Å 10603 6tw1 EMDB-10603, PDB-6tw1: Bat Influenza A polymerase termination complex with pyrophosphate using 44-mer vRNA template with mutated oligo(U) sequence Method: EM (single particle) / Resolution: 2.7 Å PDB-6tu5: Influenza A/H7N9 polymerase core (apo) Method: X-RAY DIFFRACTION / Resolution: 3.325 Å
Chemicals	ChemComp-M4H: 5-oxidanyl-4-oxidanylidene-1-[(1-pyrrolo[2,3-b]pyridin-1-ylcyclopentyl)methyl]pyridine-3-carboxylic acid ChemComp-MG: Unknown entry ChemComp-POP: PYROPHOSPHATE 2- ChemComp-HOH: WATER ChemComp-2KH: 5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine
Source	influenza a virus (a/little yellow-shouldered bat/guatemala/060/2010(h17n10)) influenza b virus synthetic construct (others) influenza a virus (a/zhejiang/dtid-zju01/2013(h7n9))
Keywords	VIRAL PROTEIN / Influenza / polymerase / viral transcription / RNA / RNA-dependent RNA polymerase