EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of the ATP-fueled ClpXP proteolytic machine bound to protein substrate.
ジャーナル・号・ページ	Elife, Vol. 9, Year 2020
掲載日	2020年2月28日
著者	Xue Fei / Tristan A Bell / Simon Jenni / Benjamin M Stinson / Tania A Baker / Stephen C Harrison / Robert T Sauer /
PubMed 要旨	ClpXP is an ATP-dependent protease in which the ClpX AAA+ motor binds, unfolds, and translocates specific protein substrates into the degradation chamber of ClpP. We present cryo-EM studies of the ...ClpXP is an ATP-dependent protease in which the ClpX AAA+ motor binds, unfolds, and translocates specific protein substrates into the degradation chamber of ClpP. We present cryo-EM studies of the enzyme that show how asymmetric hexameric rings of ClpX bind symmetric heptameric rings of ClpP and interact with protein substrates. Subunits in the ClpX hexamer assume a spiral conformation and interact with two-residue segments of substrate in the axial channel, as observed for other AAA+ proteases and protein-remodeling machines. Strictly sequential models of ATP hydrolysis and a power stroke that moves two residues of the substrate per translocation step have been inferred from these structural features for other AAA+ unfoldases, but biochemical and single-molecule biophysical studies indicate that ClpXP operates by a probabilistic mechanism in which five to eight residues are translocated for each ATP hydrolyzed. We propose structure-based models that could account for the functional results.
リンク	Elife / PubMed:32108573 / PubMed Central
手法	EM (単粒子)
解像度	3.19 - 4.28 Å
構造データ	20406 6po1 EMDB-20406, PDB-6po1: ClpX-ClpP complex bound to substrate and ATP-gamma-S, class 4 手法: EM (単粒子) / 解像度: 4.2 Å 20408 6po3 EMDB-20408, PDB-6po3: ClpX-ClpP complex bound to substrate and ATP-gamma-S, class 3 手法: EM (単粒子) / 解像度: 4.28 Å 20412 6pod EMDB-20412, PDB-6pod: ClpX-ClpP complex bound to substrate and ATP-gamma-S, class 2 手法: EM (単粒子) / 解像度: 4.05 Å 20418 6pos EMDB-20418, PDB-6pos: ClpX-ClpP complex bound to substrate and ATP-gamma-S, class 1 手法: EM (単粒子) / 解像度: 4.12 Å 20419 6pp5 EMDB-20419, PDB-6pp5: ClpX in ClpX-ClpP complex bound to substrate and ATP-gamma-S, class 4 手法: EM (単粒子) / 解像度: 3.98 Å 20420 6pp6 EMDB-20420, PDB-6pp6: ClpX in ClpX-ClpP complex bound to substrate and ATP-gamma-S, class 3 手法: EM (単粒子) / 解像度: 4.28 Å 20421 6pp7 EMDB-20421, PDB-6pp7: ClpX in ClpX-ClpP complex bound to substrate and ATP-gamma-S, class 2 手法: EM (単粒子) / 解像度: 4.05 Å 20422 6pp8 EMDB-20422, PDB-6pp8: ClpX in ClpX-ClpP complex bound to substrate and ATP-gamma-S, class 1 手法: EM (単粒子) / 解像度: 4.12 Å 20434 6ppe EMDB-20434, PDB-6ppe: ClpP and ClpX IGF loop in ClpX-ClpP complex with D7 symmetry 手法: EM (単粒子) / 解像度: 3.19 Å
化合物	ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-HOH: WATER
由来	escherichia coli (大腸菌)
キーワード	CHAPERONE / Protein degradation / AAA+ protease complex