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TitleArrangement of the Polymerase Complexes inside a Nine-Segmented dsRNA Virus.
Journal, issue, pagesStructure, Vol. 28, Issue 6, Page 604-612.e3, Year 2020
Publish dateJun 2, 2020
AuthorsJason T Kaelber / Wen Jiang / Scott C Weaver / Albert J Auguste / Wah Chiu /
PubMed AbstractMembers of the family Reoviridae package several copies of the viral polymerase complex into their capsid to carry out replication and transcription within viral particles. Classical single-particle ...Members of the family Reoviridae package several copies of the viral polymerase complex into their capsid to carry out replication and transcription within viral particles. Classical single-particle reconstruction encounters difficulties resolving structures such as the intraparticle polymerase complex because refinement can converge to an incorrect map and because the map could depict a nonrepresentative subset of particles or an average of heterogeneous particles. Using the nine-segmented Fako virus, we tested hypotheses for the arrangement and number of polymerase complexes within the virion by measuring how well each hypothesis describes the set of cryoelectron microscopy images of individual viral particles. We find that the polymerase complex in Fako virus binds at ten possible sites despite having only nine genome segments. A single asymmetric configuration describes the arrangement of these complexes in both virions and genome-free capsids. Similarities between the arrangements of Reoviridae with 9, 10, and 11 segments indicate the generalizability of this architecture.
External linksStructure / PubMed:32049031 / PubMed Central
MethodsEM (single particle)
Resolution3.9 - 11.4 Å
Structure data

EMDB-7941:
Fako virus empty particles aligned to the best decoy map (asymmetric reconstruction) showing the locations of the polymerase complexes
Method: EM (single particle)

7944

6djy

EMDB-7944: Fako virus full particles, icosahedral reconstruction
PDB-6djy: Fako virus
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-7945:
Fako virus empty particles, icosahedral reconstruction
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-7948:
Fako virus empty particles, D2-symmetrized reconstruction
Method: EM (single particle) / Resolution: 5.6 Å

EMDB-7949:
Fako virus full particles, D2-symmetrized reconstruction
Method: EM (single particle) / Resolution: 5.5 Å

EMDB-7953:
Fako virus empty particles, projection-matching reconstruction without imposed symmetry
Method: EM (single particle) / Resolution: 11.4 Å

EMDB-7954:
Fako virus full particles, projection-matching reconstruction without imposed symmetry
Method: EM (single particle) / Resolution: 10.7 Å

Source
  • fako virus
KeywordsVIRUS / capsid / virion / Reoviridae / T=2

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