EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	An MPER antibody neutralizes HIV-1 using germline features shared among donors.
ジャーナル・号・ページ	Nat Commun, Vol. 10, Issue 1, Page 5389, Year 2019
掲載日	2019年11月26日
著者	Lei Zhang / Adriana Irimia / Lingling He / Elise Landais / Kimmo Rantalainen / Daniel P Leaman / Thomas Vollbrecht / Armando Stano / Daniel I Sands / Arthur S Kim / / Pascal Poignard / Dennis R Burton / Ben Murrell / Andrew B Ward / Jiang Zhu / Ian A Wilson / Michael B Zwick /
PubMed 要旨	The membrane-proximal external region (MPER) of HIV-1 envelope glycoprotein (Env) can be targeted by neutralizing antibodies of exceptional breadth. MPER antibodies usually have long, hydrophobic ...The membrane-proximal external region (MPER) of HIV-1 envelope glycoprotein (Env) can be targeted by neutralizing antibodies of exceptional breadth. MPER antibodies usually have long, hydrophobic CDRH3s, lack activity as inferred germline precursors, are often from the minor IgG3 subclass, and some are polyreactive, such as 4E10. Here we describe an MPER broadly neutralizing antibody from the major IgG1 subclass, PGZL1, which shares germline V/D-region genes with 4E10, has a shorter CDRH3, and is less polyreactive. A recombinant sublineage variant pan-neutralizes a 130-isolate panel at 1.4 μg/ml (IC). Notably, a germline revertant with mature CDR3s neutralizes 12% of viruses and still binds MPER after DJ reversion. Crystal structures of lipid-bound PGZL1 variants and cryo-EM reconstruction of an Env-PGZL1 complex reveal how these antibodies recognize MPER and viral membrane. Discovery of common genetic and structural elements among MPER antibodies from different patients suggests that such antibodies could be elicited using carefully designed immunogens.
リンク	Nat Commun / PubMed:31772165 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.402 - 8.9 Å
構造データ	EMDB-0620: Full length HIV-1 Envelope glycoprotein clone AMC011 in complex with PGT151 Fab and PGZL1 Fab 手法: EM (単粒子) / 解像度: 8.9 Å PDB-6o3d: Crystal structure of the unbound Fab fragment of the human HIV-1 neutralizing antibody PGZL1. 手法: X-RAY DIFFRACTION / 解像度: 1.402 Å PDB-6o3g: Crystal structure of the Fab fragment of the human HIV-1 neutralizing antibody PGZL1 in complex with its MPER peptide epitope (region 671-683 of HIV-1 gp41). 手法: X-RAY DIFFRACTION / 解像度: 3.645 Å PDB-6o3j: Crystal structure of the Fab fragment of the human HIV-1 neutralizing antibody PGZL1 in complex with its MPER peptide epitope (region 671-683 of HIV-1 gp41) and phosphatidic acid (06:0 PA) 手法: X-RAY DIFFRACTION / 解像度: 3.416 Å PDB-6o3k: Crystal structure of the unbound Fab fragment of the human HIV-1 neutralizing antibody PGZL1.H4K3 手法: X-RAY DIFFRACTION / 解像度: 1.451 Å PDB-6o3l: Crystal structure of the Fab fragment of the human HIV-1 neutralizing antibody PGZL1.H4K3 in complex with its MPER peptide epitope (region 671-683 of HIV-1 gp41). 手法: X-RAY DIFFRACTION / 解像度: 1.98 Å PDB-6o3u: Crystal structure of the Fab fragment of the human HIV-1 neutralizing antibody PGZL1.H4K3 in complex with 06:0 PA 手法: X-RAY DIFFRACTION / 解像度: 3.105 Å PDB-6o41: Crystal structure of the unbound PGZL1 germline Fab fragment (PGZL1_gVmDmJ) 手法: X-RAY DIFFRACTION / 解像度: 2.465 Å PDB-6o42: Crystal structure of the germline PGZL1 (PGZL1_gVmDmJ) Fab in complex with MPER peptide epitope. 手法: X-RAY DIFFRACTION / 解像度: 2.6 Å
化合物	ChemComp-GOL: GLYCEROL / グリセロ-ル ChemComp-SO4: SULFATE ION / 硫酸ジアニオン ChemComp-HOH: WATER ChemComp-44E: (2R)-3-(phosphonooxy)propane-1,2-diyl dihexanoate / 1,2-ジカプロイル-sn-ホスファチジン酸 / リン脂質YM ChemComp-MPD: (4S)-2-METHYL-2,4-PENTANEDIOL / (S)-2-メチル-2,4-ペンタンジオ-ル / 沈殿剤YM ChemComp-PO4: PHOSPHATE ION / ホスファ-ト
由来	homo sapiens (ヒト) human immunodeficiency virus 1 (ヒト免疫不全ウイルス) streptococcus sp. group g (バクテリア)
キーワード	IMMUNE SYSTEM / PGZL1 ANTI HIV-1 / GP41 MPER / MEMBRANE LIPIDS / BROADLY NEUTRALISING HIV-1 ANTIBODY / PGZL1.H4K3 ANTI HIV-1 / germline PGZL1 ANTI HIV-1