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TitleIn vivo structure of the Legionella type II secretion system by electron cryotomography.
Journal, issue, pagesNat Microbiol, Vol. 4, Issue 12, Page 2101-2108, Year 2019
Publish dateNov 21, 2019
AuthorsDebnath Ghosal / Ki Woo Kim / Huaixin Zheng / Mohammed Kaplan / Hilary K Truchan / Alberto E Lopez / Ian E McIntire / Joseph P Vogel / Nicholas P Cianciotto / Grant J Jensen /
PubMed AbstractThe type II secretion system (T2SS) is a multiprotein envelope-spanning assembly that translocates a wide range of virulence factors, enzymes and effectors through the outer membrane of many Gram- ...The type II secretion system (T2SS) is a multiprotein envelope-spanning assembly that translocates a wide range of virulence factors, enzymes and effectors through the outer membrane of many Gram-negative bacteria. Here, using electron cryotomography and subtomogram averaging methods, we reveal the in vivo structure of an intact T2SS imaged within the human pathogen Legionella pneumophila. Although the T2SS has only limited sequence and component homology with the evolutionarily related type IV pilus (T4P) system, we show that their overall architectures are remarkably similar. Despite similarities, there are also differences, including, for example, that the T2SS-ATPase complex is usually present but disengaged from the inner membrane, the T2SS has a much longer periplasmic vestibule and it has a short-lived flexible pseudopilus. Placing atomic models of the components into our electron cryotomography map produced a complete architectural model of the intact T2SS that provides insights into the structure and function of its components, its position within the cell envelope and the interactions between its different subcomplexes.
External linksNat Microbiol / PubMed:31754273 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution40.0 - 50.0 Å
Structure data

EMDB-20712:
In vivo structure of the Legionella type II secretion system by electron cryotomography (aligning the IM-complex).
Method: EM (subtomogram averaging) / Resolution: 50.0 Å

EMDB-20713:
In vivo structure of the Legionella type II secretion system by electron cryotomography (aligning the OM-complex).
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

Source
  • Legionella pneumophila (bacteria)

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