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TitleCryo-EM Structures of Centromeric Tri-nucleosomes Containing a Central CENP-A Nucleosome.
Journal, issue, pagesStructure, Vol. 28, Issue 1, Page 44-53.e4, Year 2020
Publish dateJan 7, 2020
AuthorsYoshimasa Takizawa / Cheng-Han Ho / Hiroaki Tachiwana / Hideyuki Matsunami / Wataru Kobayashi / Midori Suzuki / Yasuhiro Arimura / Tetsuya Hori / Tatsuo Fukagawa / Melanie D Ohi / Matthias Wolf / Hitoshi Kurumizaka /
PubMed AbstractThe histone H3 variant CENP-A is a crucial epigenetic marker for centromere specification. CENP-A forms a characteristic nucleosome and dictates the higher-order configuration of centromeric ...The histone H3 variant CENP-A is a crucial epigenetic marker for centromere specification. CENP-A forms a characteristic nucleosome and dictates the higher-order configuration of centromeric chromatin. However, little is known about how the CENP-A nucleosome affects the architecture of centromeric chromatin. In this study, we reconstituted tri-nucleosomes mimicking a centromeric nucleosome arrangement containing the CENP-A nucleosome, and determined their 3D structures by cryoelectron microscopy. The H3-CENP-A-H3 tri-nucleosomes adopt an untwisted architecture, with an outward-facing linker DNA path between nucleosomes. This is distinct from the H3-H3-H3 tri-nucleosome architecture, with an inward-facing DNA path. Intriguingly, the untwisted architecture may allow the CENP-A nucleosome to be exposed to the solvent in the condensed chromatin model. These results provide a structural basis for understanding the 3D configuration of CENP-A-containing chromatin, and may explain how centromeric proteins can specifically target the CENP-A nucleosomes buried in robust amounts of H3 nucleosomes in centromeres.
External linksStructure / PubMed:31711756
MethodsEM (single particle)
Resolution12.3 - 19.6 Å
Structure data

EMDB-0768: H3-CENP-A-H3 tri-nucleosome with the 22 base-pair linker DNA
PDB-6l49: H3-CA-H3 tri-nucleosome with the 22 base-pair linker DNA
Method: EM (single particle) / Resolution: 18.7 Å

EMDB-0769:
H3-CENP-A-H3 tri-nucleosome with the 30 base-pair linker DNA
Method: EM (single particle) / Resolution: 19.6 Å

EMDB-0770: H3-H3-H3 tri-nucleosome with the 22 base-pair linker DNA, Class1
PDB-6l4a: H3-H3-H3 tri-nucleosome with the 22 base-pair linker DNA
Method: EM (single particle) / Resolution: 12.3 Å

EMDB-0771:
H3-H3-H3 tri-nucleosome with the 22 base-pair linker DNA, Class2
Method: EM (single particle) / Resolution: 15.1 Å

EMDB-0772:
H3-H3-H3 tri-nucleosome with the 30 base-pair linker DNA
Method: EM (single particle) / Resolution: 15.7 Å

Source
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsCENPA protein, human / Centromere Protein A / Cryoelectron Microscopy / Histones / Humans / Models, Molecular / Protein Binding / Protein Conformation / NUCLEAR PROTEIN / Chromatin / Nucleosome / Centromere

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