EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Conservative transcription in three steps visualized in a double-stranded RNA virus.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 26, Issue 11, Page 1023-1034, Year 2019
掲載日	2019年11月6日
著者	Yanxiang Cui / Yinong Zhang / Kang Zhou / Jingchen Sun / Z Hong Zhou /
PubMed 要旨	Endogenous RNA transcription characterizes double-stranded RNA (dsRNA) viruses in the Reoviridae, a family that is exemplified by its simple, single-shelled member cytoplasmic polyhedrosis virus (CPV) ...Endogenous RNA transcription characterizes double-stranded RNA (dsRNA) viruses in the Reoviridae, a family that is exemplified by its simple, single-shelled member cytoplasmic polyhedrosis virus (CPV). Because of the lack of in situ structures of the intermediate stages of RNA-dependent RNA polymerase (RdRp) during transcription, it is poorly understood how RdRp detects environmental cues and internal transcriptional states to initiate and coordinate repeated cycles of transcript production inside the capsid. Here, we captured five high-resolution (2.8-3.5 Å) RdRp-RNA in situ structures-representing quiescent, initiation, early elongation, elongation and abortive states-under seven experimental conditions of CPV. We observed the 'Y'-form initial RNA fork in the initiation state and the complete transcription bubble in the elongation state. These structures reveal that de novo RNA transcription involves three major conformational changes during state transitions. Our results support an ouroboros model for endogenous conservative transcription in dsRNA viruses.
リンク	Nat Struct Mol Biol / PubMed:31695188 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 4.1 Å
構造データ	20581 6ty8 EMDB-20581, PDB-6ty8: In situ structure of BmCPV RNA dependent RNA polymerase at quiescent state 手法: EM (単粒子) / 解像度: 3.0 Å 20582 6ty9 EMDB-20582, PDB-6ty9: In situ structure of BmCPV RNA dependent RNA polymerase at initiation state 手法: EM (単粒子) / 解像度: 2.9 Å 20585 6tz0 EMDB-20585, PDB-6tz0: In situ structure of BmCPV RNA-dependent RNA polymerase at abortive state 手法: EM (単粒子) / 解像度: 2.8 Å 20586 6tz1 EMDB-20586, PDB-6tz1: In situ structure of BmCPV RNA-dependent RNA polymerase at early-elongation state 手法: EM (単粒子) / 解像度: 3.4 Å 20587 6tz2 EMDB-20587, PDB-6tz2: In situ structure of BmCPV RNA-dependent RNA polymerase at elongation state 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-20595: BmCPV virion 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-20596: BmCPV virion with SAM 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-20597: BmCPV virion with SAM and GTP 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-20598: BmCPV virion with SAM and ATP 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-20599: BmCPV virion with SAM, GTP, and ATP 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-20600: BmCPV virion with SAM, GTP, ATP, and UTP 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-20601: BmCPV virion with SAM, GTP, ATP, UTP, and CTP 手法: EM (単粒子) / 解像度: 4.1 Å
化合物	ChemComp-GTA: P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE / 7-メチルグアノシン-7-イウム5′-三りん酸O^γ-(5′-アデノシル) ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-UTP: URIDINE 5'-TRIPHOSPHATE / UTP / UTPYM
由来	bombyx mori cytoplasmic polyhedrosis virus (ウイルス)
キーワード	VIRAL PROTEIN / TRANSFERASE / RdRp / TRANSFERASE/RNA / RdRp-RNA complex / Initiation / Unwinding / Cap-binding / TRANSFERASE-RNA complex / Early-elongation / Elongation / Transcription Bubble