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Structure paper

TitleTemporal Ordering in Endocytic Clathrin-Coated Vesicle Formation via AP2 Phosphorylation.
Journal, issue, pagesDev. Cell, Vol. 50, Page 494-508.e11, Year 2019
Publish dateJan 15, 2019 (structure data deposition date)
AuthorsWrobel, A.G. / Kadlecova, Z. / Kamenicky, J. / Yang, J.C. / Herrmann, T. / Kelly, B.T. / McCoy, A.J. / Evans, P.R. / Martin, S. / Muller, S. ...Wrobel, A.G. / Kadlecova, Z. / Kamenicky, J. / Yang, J.C. / Herrmann, T. / Kelly, B.T. / McCoy, A.J. / Evans, P.R. / Martin, S. / Muller, S. / Sroubek, F. / Neuhaus, D. / Honing, S. / Owen, D.J.
External linksDev. Cell / PubMed:31430451
MethodsX-ray diffraction / NMR (solution)
Resolution2.56 - 5 Å
Structure data

PDB-6qh5:
AP2 clathrin adaptor mu2T156-phosphorylated core in closed conformation
Method: X-RAY DIFFRACTION / Resolution: 2.56 Å

PDB-6qh6:
AP2 clathrin adaptor core with two cargo peptides in open+ conformation
Method: X-RAY DIFFRACTION / Resolution: 5 Å

PDB-6qh7:
AP2 clathrin adaptor mu2T156-phosphorylated core with two cargo peptides in open+ conformation
Method: X-RAY DIFFRACTION / Resolution: 3.4 Å

PDB-6rh5:
Solution structure and 1H, 13C and 15N chemical shift assignments for NECAP1 PHear domain
Method: SOLUTION NMR

PDB-6rh6:
Solution structure and 1H, 13C and 15N chemical shift assignments for the complex of NECAP1 PHear domain with phosphorylated AP2 mu2 148-163
Method: SOLUTION NMR

Chemicals

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

Source
  • rattus norvegicus (Norway rat)
  • homo sapiens (human)
  • mus musculus (house mouse)
  • bos taurus (domestic cattle)
KeywordsPROTEIN TRANSPORT / ENDOCYTOSIS / PHOSPHORYLATION / CELL MEMBRANE / TRAFFICKING / clathrin mediated endocytosis / regulation by phosphorylation / AP2 endocytic adaptor / NECAP SNX9 / NECAP / SNX9

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