Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Cryo-EM structures capture the transport cycle of the P4-ATPase flippase.
Journal, issue, pages	Science, Vol. 365, Issue 6458, Page 1149-1155, Year 2019
Publish date	Sep 13, 2019
Authors	Masahiro Hiraizumi / Keitaro Yamashita / Tomohiro Nishizawa / Osamu Nureki /
PubMed Abstract	In eukaryotic membranes, type IV P-type adenosine triphosphatases (P4-ATPases) mediate the translocation of phospholipids from the outer to the inner leaflet and maintain lipid asymmetry, which is ...In eukaryotic membranes, type IV P-type adenosine triphosphatases (P4-ATPases) mediate the translocation of phospholipids from the outer to the inner leaflet and maintain lipid asymmetry, which is critical for membrane trafficking and signaling pathways. Here, we report the cryo-electron microscopy structures of six distinct intermediates of the human ATP8A1-CDC50a heterocomplex at resolutions of 2.6 to 3.3 angstroms, elucidating the lipid translocation cycle of this P4-ATPase. ATP-dependent phosphorylation induces a large rotational movement of the actuator domain around the phosphorylation site in the phosphorylation domain, accompanied by lateral shifts of the first and second transmembrane helices, thereby allowing phosphatidylserine binding. The phospholipid head group passes through the hydrophilic cleft, while the acyl chain is exposed toward the lipid environment. These findings advance our understanding of the flippase mechanism and the disease-associated mutants of P4-ATPases.
External links	Science / PubMed:31416931
Methods	EM (single particle)
Resolution	2.63 - 3.42 Å
Structure data	9931 6k7g EMDB-9931, PDB-6k7g: Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1 state class1) Method: EM (single particle) / Resolution: 3.3 Å 9932 6k7h EMDB-9932, PDB-6k7h: Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1 state class2) Method: EM (single particle) / Resolution: 3.22 Å EMDB-9933: Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1 state class3) Method: EM (single particle) / Resolution: 3.42 Å 9934 6k7i EMDB-9934, PDB-6k7i: Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1-ATP state class2) Method: EM (single particle) / Resolution: 3.22 Å 9935 6k7j EMDB-9935, PDB-6k7j: Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1-ATP state class1) Method: EM (single particle) / Resolution: 3.08 Å EMDB-9936: Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1-ATP state class3) Method: EM (single particle) / Resolution: 3.32 Å 9937 6k7k EMDB-9937, PDB-6k7k: Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1-ADP-Pi state) Method: EM (single particle) / Resolution: 3.04 Å EMDB-9938: Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E2P state class1) Method: EM (single particle) / Resolution: 2.76 Å 9939 6k7l EMDB-9939, PDB-6k7l: Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E2P state class2) Method: EM (single particle) / Resolution: 2.83 Å EMDB-9940: Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E2P state class3) Method: EM (single particle) / Resolution: 2.63 Å 9941 6k7m EMDB-9941, PDB-6k7m: Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E2Pi-PL state) Method: EM (single particle) / Resolution: 2.95 Å 9942 6k7n EMDB-9942, PDB-6k7n: Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1P state) Method: EM (single particle) / Resolution: 2.84 Å
Chemicals	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine ChemComp-Y01: CHOLESTEROL HEMISUCCINATE ChemComp-ACP: PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / AMP-PCP, energy-carrying molecule analogueYM ChemComp-MG: Unknown entry ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM / Adenosine diphosphate ChemComp-ALF: TETRAFLUOROALUMINATE ION ChemComp-BEF: BERYLLIUM TRIFLUORIDE ION ChemComp-D39: (2~{S})-2-azanyl-3-[[(2~{R})-3-hexadecanoyloxy-2-[(~{Z})-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-propanoic acid
Source	homo sapiens (human)
Keywords	MEMBRANE PROTEIN / flippase