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-Structure paper
タイトル | Resting-State Structure and Gating Mechanism of a Voltage-Gated Sodium Channel. |
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ジャーナル・号・ページ | Cell, Vol. 178, Issue 4, Page 993-1003.e12, Year 2019 |
掲載日 | 2019年8月8日 |
著者 | Goragot Wisedchaisri / Lige Tonggu / Eedann McCord / Tamer M Gamal El-Din / Liguo Wang / Ning Zheng / William A Catterall / |
PubMed 要旨 | Voltage-gated sodium (Na) channels initiate action potentials in nerve, muscle, and other electrically excitable cells. The structural basis of voltage gating is uncertain because the resting state ...Voltage-gated sodium (Na) channels initiate action potentials in nerve, muscle, and other electrically excitable cells. The structural basis of voltage gating is uncertain because the resting state exists only at deeply negative membrane potentials. To stabilize the resting conformation, we inserted voltage-shifting mutations and introduced a disulfide crosslink in the VS of the ancestral bacterial sodium channel NaAb. Here, we present a cryo-EM structure of the resting state and a complete voltage-dependent gating mechanism. The S4 segment of the VS is drawn intracellularly, with three gating charges passing through the transmembrane electric field. This movement forms an elbow connecting S4 to the S4-S5 linker, tightens the collar around the S6 activation gate, and prevents its opening. Our structure supports the classical "sliding helix" mechanism of voltage sensing and provides a complete gating mechanism for voltage sensor function, pore opening, and activation-gate closure based on high-resolution structures of a single sodium channel protein. |
リンク | Cell / PubMed:31353218 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 2.75 - 4.0 Å |
構造データ | EMDB-20265, PDB-6p6w: PDB-6p6x: PDB-6p6y: |
化合物 | ChemComp-PX4: ChemComp-CPS: |
由来 |
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キーワード | membrane protein / metal transport / Ion channel / ion transport protein |