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-Structure paper
Title | The structure of the yeast Ctf3 complex. |
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Journal, issue, pages | Elife, Vol. 8, Year 2019 |
Publish date | Jun 13, 2019 |
Authors | Stephen M Hinshaw / Andrew N Dates / Stephen C Harrison / |
PubMed Abstract | Kinetochores are the chromosomal attachment points for spindle microtubules. They are also signaling hubs that control major cell cycle transitions and coordinate chromosome folding. Most well- ...Kinetochores are the chromosomal attachment points for spindle microtubules. They are also signaling hubs that control major cell cycle transitions and coordinate chromosome folding. Most well-studied eukaryotes rely on a conserved set of factors, which are divided among two loosely-defined groups, for these functions. Outer kinetochore proteins contact microtubules or regulate this contact directly. Inner kinetochore proteins designate the kinetochore assembly site by recognizing a specialized nucleosome containing the H3 variant Cse4/CENP-A. We previously determined the structure, resolved by cryo-electron microscopy (cryo-EM), of the yeast Ctf19 complex (Ctf19c, homologous to the vertebrate CCAN), providing a high-resolution view of inner kinetochore architecture (Hinshaw and Harrison, 2019). We now extend these observations by reporting a near-atomic model of the Ctf3 complex, the outermost Ctf19c sub-assembly seen in our original cryo-EM density. The model is sufficiently well-determined by the new data to enable molecular interpretation of Ctf3 recruitment and function. |
External links | Elife / PubMed:31194673 / PubMed Central |
Methods | EM (single particle) |
Resolution | 4.18 Å |
Structure data | EMDB-20200, PDB-6oua: |
Source |
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Keywords | CELL CYCLE / kinetochore / chromosome / cryo-em |