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TitleThe structural basis for release-factor activation during translation termination revealed by time-resolved cryogenic electron microscopy.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 2579, Year 2019
Publish dateJun 12, 2019
AuthorsZiao Fu / Gabriele Indrisiunaite / Sandip Kaledhonkar / Binita Shah / Ming Sun / Bo Chen / Robert A Grassucci / Måns Ehrenberg / Joachim Frank /
PubMed AbstractWhen the ribosome encounters a stop codon, it recruits a release factor (RF) to hydrolyze the ester bond between the peptide chain and tRNA. RFs have structural motifs that recognize stop codons in ...When the ribosome encounters a stop codon, it recruits a release factor (RF) to hydrolyze the ester bond between the peptide chain and tRNA. RFs have structural motifs that recognize stop codons in the decoding center and a GGQ motif for induction of hydrolysis in the peptidyl transfer center 70 Å away. Surprisingly, free RF2 is compact, with only 20 Å between its codon-reading and GGQ motifs. Cryo-EM showed that ribosome-bound RFs have extended structures, suggesting that RFs are compact when entering the ribosome and then extend their structures upon stop codon recognition. Here we use time-resolved cryo-EM to visualize transient compact forms of RF1 and RF2 at 3.5 and 4 Å resolution, respectively, in the codon-recognizing ribosome complex on the native pathway. About 25% of complexes have RFs in the compact state at 24 ms reaction time, and within 60 ms virtually all ribosome-bound RFs are transformed to their extended forms.
External linksNat Commun / PubMed:31189921 / PubMed Central
MethodsEM (single particle)
Resolution2.9 - 4.2 Å
Structure data

20173

6ore

EMDB-20173, PDB-6ore:
Release complex 70S
Method: EM (single particle) / Resolution: 2.9 Å

20174

6orl

EMDB-20174, PDB-6orl:
RF1 pre-accommodated 70S complex at 24 ms
Method: EM (single particle) / Resolution: 3.5 Å

20184

6osk

EMDB-20184, PDB-6osk:
RF1 accommodated 70S complex at 60 ms
Method: EM (single particle) / Resolution: 3.6 Å

20187

6osq

EMDB-20187, PDB-6osq:
RF1 accommodated state bound Release complex 70S at long incubation time point
Method: EM (single particle) / Resolution: 3.5 Å

20188

6ost

EMDB-20188, PDB-6ost:
RF2 pre-accommodated state bound Release complex 70S at 24ms
Method: EM (single particle) / Resolution: 4.2 Å

20193

6ot3

EMDB-20193, PDB-6ot3:
RF2 accommodated state bound Release complex 70S at 24 ms
Method: EM (single particle) / Resolution: 3.9 Å

20204

6ouo

EMDB-20204, PDB-6ouo:
RF2 accommodated state bound 70S complex at long incubation time
Method: EM (single particle) / Resolution: 3.7 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • escherichia coli (E. coli)
  • Escherichia coli
KeywordsRIBOSOME / Time-resolved cryo-EM / Termination / short-lived / millisecond

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