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Structure paper

TitleLong-wavelength native-SAD phasing: opportunities and challenges.
Journal, issue, pagesIucrj, Vol. 6, Page 373-386, Year 2019
Publish dateNov 13, 2018 (structure data deposition date)
AuthorsBasu, S. / Olieric, V. / Leonarski, F. / Matsugaki, N. / Kawano, Y. / Takashi, T. / Huang, C.Y. / Yamada, Y. / Vera, L. / Olieric, N. ...Basu, S. / Olieric, V. / Leonarski, F. / Matsugaki, N. / Kawano, Y. / Takashi, T. / Huang, C.Y. / Yamada, Y. / Vera, L. / Olieric, N. / Basquin, J. / Wojdyla, J.A. / Bunk, O. / Diederichs, K. / Yamamoto, M. / Wang, M.
External linksIucrj / PubMed:31098019
MethodsX-ray diffraction
Resolution2.95 Å
Structure data

PDB-6i59:
Long wavelength native-SAD phasing of Sen1 helicase
Method: X-RAY DIFFRACTION / Resolution: 2.95 Å

PDB-6i5c:
Long wavelength native-SAD phasing of Tubulin-Stathmin-TTL complex
Method: X-RAY DIFFRACTION / Resolution: 2.95 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-GOL:
GLYCEROL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-CA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-ACP:
PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / AMP-PCP, energy-carrying molecule analogue*YM

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • bos taurus (domestic cattle)
  • rattus norvegicus (Norway rat)
  • gallus gallus (chicken)
KeywordsDNA BINDING PROTEIN / Sen1 helicase / Super family 1B / Long-wavelength native-SAD phasing / native-SAD / S-SAD / STRUCTURAL PROTEIN / Tubulin complex / T2R-TTL

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