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Structure paper

TitleStructure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate.
Journal, issue, pagesNat Commun, Vol. 10, Page 1717-1717, Year 2019
Publish dateJan 10, 2019 (structure data deposition date)
AuthorsPalm, G.J. / Reisky, L. / Bottcher, D. / Muller, H. / Michels, E.A.P. / Walczak, M.C. / Berndt, L. / Weiss, M.S. / Bornscheuer, U.T. / Weber, G.
External linksNat Commun / PubMed:30979881
MethodsX-ray diffraction
Resolution2 - 2.2 Å
Structure data

PDB-6qg9:
Crystal structure of Ideonella sakaiensis MHETase
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

PDB-6qga:
Crystal structure of Ideonella sakaiensis MHETase bound to the non-hydrolyzable ligand MHETA
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-6qgb:
Crystal structure of Ideonella sakaiensis MHETase bound to benzoic acid
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-6qgc:
PETase from Ideonella sakaiensis without ligand
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL / Ethylene glycol

ChemComp-CA:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-ACT:
ACETATE ION / Acetate

ChemComp-HOH:
WATER / Water

ChemComp-J1K:
4-(2-hydroxyethylcarbamoyl)benzoic acid

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM / 2-Methyl-2,4-pentanediol

ChemComp-CL:
Unknown entry / Chloride

ChemComp-BEZ:
BENZOIC ACID / Benzoic acid

Source
  • ideonella sakaiensis (strain 201-f6) (bacteria)
  • ideonella sakaiensis (bacteria)
KeywordsHYDROLASE / Plastic-degrading hydrolase / alpha/beta hydrolase fold / I. sakaiensis catalytic triad / PET degradation

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